Proteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors
| Autor: | Hélène Omer, Béatrice Alpha-Bazin, Catherine Duport, Jean-Luc Brunet, Jean Armengaud |
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| Přispěvatelé: | Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Abeilles et environnement (AE), Institut National de la Recherche Agronomique (INRA), Duport, Catherine |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
[SDV]Life Sciences [q-bio] Mutant Bacillus cereus lcsh:QR1-502 Virulence structure cellulaire Proteomics Cell morphology exoproteome Microbiology lcsh:Microbiology comparative proteomics protéine sécrétée [SDV.IDA]Life Sciences [q-bio]/Food engineering [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering protéome Original Research biology acide gras cellular proteome souche mutante cytotoxicité microscopie électronique biology.organism_classification Proteogenomics virulence Cereus Proteome metabolism RNA-seq mobilité |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2015, 6, 15 p. ⟨10.3389/fmicb.2015.01004⟩ Frontiers in Microbiology, 2015, 6, 15 p. ⟨10.3389/fmicb.2015.01004⟩ Frontiers in Microbiology (6), 15 p.. (2015) Frontiers in Microbiology, Vol 6 (2015) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2015.01004⟩ |
| Popis: | International audience; Bacillus cereus is a Gram positive pathogen that causes a wide variety of diseases in humans. It secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. EntD is a novel exoprotein identified by proteogenomics of B.cereus ATCC 14579. We constructed a λentD mutant and analyzed the impact of entD disruption on the cellular proteome and exoproteome isolated from early, late, and stationary-phase cultures. We identified 308 and 79 proteins regulated by EntD in the cellular proteome and the exoproteome, respectively. The contribution of these proteins to important virulence-associated functions, including central metabolism, cell structure, antioxidative ability, cell motility, and toxin production, are presented. The proteomic data were correlated with the growth defect, cell morphology change, reduced motility, and reduced cytotoxicity of the λentD mutant strain. We conclude that EntD is an important player in B.cereus virulence. The function of EntD and the putative EntD-dependent regulatory network are discussed. To our knowledge, this study is the first characterization of an Ent family protein in a species of the B.cereus group. |
| Databáze: | OpenAIRE |
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