Inhibition of osteoblastic cell differentiation by conditioned medium derived from the human prostatic cancer cell line PC-3 in vitro
| Autor: | Toshihiko Nagata, Masatoshi Kataoka, Akihiko Okuyama, Seiji Nishikawa, Hiroyuki Kadono, Teruo Nakamura, Jun-ichi Kido, Dai Ikedo, Noriyuki Yamauchi, Norio Nonomura, Akemichi Ueno, Keiji Ohishi |
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| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Sialoglycoproteins Osteocalcin Cell Gene Expression Biology Bone tissue Biochemistry Bone and Bones Bone remodeling Internal medicine Tumor Cells Cultured medicine Animals Humans RNA Messenger Osteopontin Molecular Biology Osteosarcoma Osteoblasts Cell growth Prostatic Neoplasms Bone metastasis Cell Differentiation Cell Biology Alkaline Phosphatase Embryo Mammalian medicine.disease Rats medicine.anatomical_structure Endocrinology Cell culture Culture Media Conditioned biology.protein Cancer research Cell Division |
| Zdroj: | Journal of Cellular Biochemistry. 67:248-256 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/(sici)1097-4644(19971101)67:2<248::aid-jcb10>3.0.co;2-b |
| Popis: | Human prostatic carcinoma frequently metastasizes to bone tissue and activates bone metabolism, especially bone formation, at the site of metastasis. It has been reported that an extract of prostatic carcinoma and conditioned medium (CM) of a human prostatic carcinoma cell line, PC-3, established from a bone metastastic lesion, stimulate osteoblastic cell proliferation. However, there is little information about the effect of PC-3 CM on the differentiation of osteoblastic cells. In this study, we investigated the effect of PC-3 CM on the differentiation of two types of osteoblastic cells, primary fetal rat calvaria (RC) cells containing many undifferentiated osteoprogenitor cells, and ROS 17/2.8, a well-differentiated rat osteosarcoma cell line. PC-3 CM inhibited bone nodule formation and the activity of alkaline phosphatase (ALPase), an osteoblastic marker enzyme, on days 7, 14, and 21 (RC cells) or 3, 6, and 9 (ROS 17/2.8 cells) in a dose-dependent manner (5–30% CM). However, the CM did not affect cell proliferation or cell viability. PC-3 CM was found to markedly block the gene expression of ALPase and osteocalcin (OCN) mRNAs but had no effect on the mRNA expression of osteopontin (OPN), the latter two being noncollagenous proteins related to bone matrix mineralization. These findings suggest that PC-3 CM contains a factor that inhibits osteoblastic cell differentiation and that this factor may be involved in the process of bone metastasis from prostatic carcinoma. J. Cell. Biochem. 67:248–256, 1997. © 1997 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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