Characterization of the inhibition of hepatitis C virus entry by in vitro-generated and patient-derived oxidized low-density lipoprotein
| Autor: | Ilka Wappler, Stefanie Ernst, Christoph Sarrazin, Sandra Westhaus, Maren Luchtefeld, Dorothea Bankwitz, Thomas Pietschmann, Michael P. Manns, Clemens Agné, Sandra Ciesek, Thomas von Hahn, Katrin Rohrmann, Bernhard Schieffer |
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| Přispěvatelé: | Institute for Molecular Biology, Medizinische Hochschule Hannover, Hannover, Germany. |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
CD36 Antigens
Carcinoma Hepatocellular Genotype Hepatitis C virus Context (language use) Endogeny Hepacivirus In Vitro Techniques Biology Antigens CD36 medicine.disease_cause Virus Replication Cell Line Tumor medicine Humans Scavenger receptor Cells Cultured Hepatology Liver Neoplasms Virion virus diseases Hepatitis C Chronic Viral Load Virology digestive system diseases Entry inhibitor Lipoproteins LDL Viral replication DNA Viral lipids (amino acids peptides and proteins) Viral load Lipoprotein medicine.drug |
| Popis: | Oxidized low-density lipoprotein (oxLDL) has been reported as an inhibitor of hepatitis C virus (HCV) cell entry, making it the only known component of human lipid metabolism with an antiviral effect on HCV. However, several questions remain open, including its effect on full-length cell-culture–grown HCV (HCVcc) of different genotypes or on other steps of the viral replication cycle, its mechanism of action, and whether endogenous oxLDL shares the anti-HCV properties of in vitro–generated oxLDL. We combined molecular virology tools with oxLDL serum measurements in different patient cohorts to address these questions. We found that oxLDL inhibits HCVcc at least as potently as HCV pseudoparticles. There was moderate variation between genotypes, with genotype 4 appearing the most oxLDL sensitive. Intracellular RNA replication and assembly and release of new particles were unaffected. HCV particles entering target cells lost oxLDL sensitivity with time kinetics parallel to anti-SR-BI (scavenger receptor class B type I), but significantly earlier than anti-CD81, suggesting that oxLDL acts by perturbing interaction between HCV and SR-BI. Finally, in chronically HCV-infected individuals, endogenous serum oxLDL levels did not correlate with viral load, but in HCV-negative sera, high endogenous oxLDL had a negative effect on HCV infectivity in vitro. Conclusion: oxLDL is a potent pangenotype HCV entry inhibitor that maintains its activity in the context of human serum and targets an early step of HCV entry. (HEPATOLOGY 2013) |
| Databáze: | OpenAIRE |
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