Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
| Autor: | William J. Roberts, Li Zuo, Kevin D. Evans |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Lung General Immunology and Microbiology Diagnostic ultrasound business.industry General Chemical Engineering General Neuroscience Diaphragm Respiratory Tract Diseases Ultrasound General Biochemistry Genetics and Molecular Biology Diaphragm (structural system) Contractility Mouse Diaphragm Mice medicine.anatomical_structure In vivo Animals Medicine Longitudinal Studies Respiratory system business Ultrasonography |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/51290 |
| Popis: | Function analysis of rodent respiratory skeletal muscles, particularly the diaphragm, is commonly performed by isolating muscle strips using invasive surgical procedures. Although this is an effective method of assessing in vitro diaphragm activity, it involves non-survival surgery. The application of non-invasive ultrasound imaging as an in vivo procedure is beneficial since it not only reduces the number of animals sacrificed, but is also suitable for monitoring disease progression in live mice. Thus, our ultrasound imaging method may likely assist in the development of novel therapies that alleviate muscle injury induced by various respiratory diseases. Particularly, in clinical diagnoses of obstructive lung diseases, ultrasound imaging has the potential to be used in conjunction with other standard tests to detect the early onset of diaphragm muscle fatigue. In the current protocol, we describe how to accurately evaluate diaphragm contractility in a mouse model using a diagnostic ultrasound imaging technique. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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