Pipette-tip micro-solid phase extraction based on melamine-foam@polydopamine followed by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry for detection of psychotropic drugs in human serum
| Autor: | Ling Wang, Xiaodan He, Xuehua Jiang, Tangqiang Sun |
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| Rok vydání: | 2020 |
| Předmět: |
Indoles
Polymers Clinical Biochemistry Mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Adsorption Column chromatography X-ray photoelectron spectroscopy Limit of Detection Desorption Humans Solid phase extraction Chromatography High Pressure Liquid Detection limit Psychotropic Drugs Chromatography Chemistry Triazines 010401 analytical chemistry Extraction (chemistry) Solid Phase Extraction Reproducibility of Results Cell Biology General Medicine 0104 chemical sciences Linear Models |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1163 |
| ISSN: | 1873-376X |
| Popis: | In this work, pipette-tip micro-solid phase extraction (PT-μSPE) which packed by melamine-foam@polydopamine (MF@PDA) coupled with ultra-high-performance liquid chromatography–quadrupole–time-of-flight mass spectrometry (UHPLC-QTOF) was developed for extraction and determination of psychotropic drugs in serum samples. Considering the operation back pressure, the melamine-foam as carrier material with 3D cross-linked grid structure can provide high permeability and contact surface. MF@PDA was prepared by self-polymerization reaction of dopamine under weak alkaline conditions and characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS). The surface group of PDA containing catechol structure, quinone structure and amine group has multi-interaction with psychotropic drugs which can increase the adsorption capacity. Moreover, the parameters affecting extraction efficiencies such as extraction and desorption cycle, pH value, eluent type, ionic strength and amount of sorbent were investigated. Based on the high sensitivity and accuracy mass measurement by TOF/MS, under the optimized extraction condition, the limits of detection (LOD) of this method were obtained in the range of 0.002–0.1 ng ml−1. The linearity was ranged from 0.01 ng ml−1 to 600 ng ml−1, and all the correlation coefficients (R2) were above 0.993. The spiked recoveries were in the range of 80.04% to 109.18% in real sample test and RSD values obtained from 0.95% to 9.85%. The results demonstrate that MF@PDA-PT-μSPE-UHPLC-QTOF is a sample and reliable method for the detection of psychotropic drugs in serum sample. |
| Databáze: | OpenAIRE |
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