Virus-encoded microRNA contributes to the molecular profile of EBV-positive Burkitt lymphomas
| Autor: | Cristiana Bellan, Emily A Rogena, Maria Rosaria Sapienza, Maria Raffaella Ambrosio, Davide Gibellini, Stefano Lazzi, Lynnette K Tumwine, Maria Antonella Laginestra, Mohsen Navari, Giulia De Falco, Fabio Fuligni, Pier Paolo Piccaluga, Jessica Consiglio, Maura Rossi, Maryam Etebari, Carlo M. Croce, Lorenzo Leoncini, Stefano Pileri, Claudio Tripodo |
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| Přispěvatelé: | Piccaluga, P., Navari, M., De Falco, G., Ambrosio, M., Lazzi, S., Fuligni, F., Bellan, C., Rossi, M., Sapienza, M., Laginestra, M., Etebari, M., Rogena, E., Tumwine, L., Tripodo, C., Gibellini, D., Consiglio, J., Croce, C., Pileri, S., Leoncini, L., Piccaluga, Pier Paolo, Navari, Mohsen, De Falco, Giulia, Ambrosio, Maria Raffaella, Lazzi, Stefano, Fuligni, Fabio, Bellan, Cristiana, Rossi, Maura, Sapienza, Maria Rosaria, Laginestra, Maria Antonella, Etebari, Maryam, Rogena, Emily A., Tumwine, Lynnette, Tripodo, Claudio, Gibellini, Davide, Consiglio, Jessica, Croce, Carlo M., Pileri, Stefano A., Leoncini, Lorenzo |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
BART6 Burkitt lymphoma EBV miRNA pathogenesis Epstein-Barr Virus Infections Herpesvirus 4 Human pathogenesi RNA-binding protein RNA-Binding Protein Epstein-Barr Virus Infection hemic and lymphatic diseases Cluster Analysis Viral Oligonucleotide Array Sequence Analysis Genetics Burkitt Lymphoma Cytoskeletal Proteins Gene Expression Profiling Gene Expression Regulation Neoplastic Gene Expression Regulation Viral Host-Pathogen Interactions Humans Immunohistochemistry MicroRNAs Neoplasm Proteins Phospholipase C delta RNA Viral RNA-Binding Proteins Reverse Transcriptase Polymerase Chain Reaction ras Proteins Oncology Microfilament Proteins Intracellular Signaling Peptides and Proteins MicroRNA Phenotype Host-Pathogen Interaction Human Research Paper Biology Settore MED/08 - Anatomia Patologica Virus Neoplasm Protein 03 medical and health sciences microRNA Cytoskeletal Protein medicine Epstein–Barr virus infection Gene Neoplastic Cluster Analysi Oligonucleotide Array Sequence Analysi Herpesvirus 4 ras Protein medicine.disease Lymphoma Gene expression profiling 030104 developmental biology Gene Expression Regulation RNA burkitt lymphoma |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Burkitt lymphoma (BL) is an aggressive neoplasm characterized by consistent morphology and phenotype, typical clinical behavior and distinctive molecular profile. The latter is mostly driven by the MYC over-expression associated with the characteristic translocation (8;14) (q24; q32) or with variant lesions. Additional genetic events can contribute to Burkitt Lymphoma pathobiology and retain clinical significance. A pathogenetic role for Epstein-Barr virus infection in Burkitt lymphomagenesis has been suggested; however, the exact function of the virus is largely unknown. In this study, we investigated the molecular profiles (genes and microRNAs) of Epstein-Barr virus-positive and -negative BL, to identify specific patterns relying on the differential expression and role of Epstein-Barr virus-encoded microRNAs. First, we found significant differences in the expression of viral microRNAs and in selected target genes. Among others, we identified LIN28B, CGNL1, GCET2, MRAS, PLCD4, SEL1L, SXX1, and the tyrosine kinases encoding STK10/STK33, all provided with potential pathogenetic significance. GCET2, also validated by immunohistochemistry, appeared to be a useful marker for distinguishing EBV-positive and EBV-negative cases. Further, we provided solid evidences that the EBV-encoded microRNAs (e.g. BART6) significantly mold the transcriptional landscape of Burkitt Lymphoma clones. In conclusion, our data indicated significant differences in the transcriptional profiles of EBV-positive and EBV-negative BL and highlight the role of virus encoded miRNA. |
| Databáze: | OpenAIRE |
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