A complex containing the O-GlcNAc transferase OGT-1 and the ubiquitin ligase EEL-1 regulates GABA neuron function
| Autor: | Marissa J. Maroni, Andrew C. Giles, Brock Grill, Karla J. Opperman, Rubens Tavora, Muriel Desbois |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proteomics endocrine system animal structures Ubiquitin-Protein Ligases Presynaptic Terminals Neurotransmission Inhibitory postsynaptic potential N-Acetylglucosaminyltransferases Biochemistry Synaptic Transmission Chromatography Affinity Synapse 03 medical and health sciences Neurobiology medicine Animals GABAergic Neurons Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology 030102 biochemistry & molecular biology biology Chemistry Cell Biology Motor neuron biology.organism_classification Cell biology Ubiquitin ligase 030104 developmental biology medicine.anatomical_structure biology.protein GABAergic Neuron Aldicarb Protein Binding Signal Transduction |
| Popis: | Inhibitory GABAergic transmission is required for proper circuit function in the nervous system. However, our understanding of molecular mechanisms that preferentially influence GABAergic transmission, particularly presynaptic mechanisms, remains limited. We previously reported that the ubiquitin ligase EEL-1 preferentially regulates GABAergic presynaptic transmission. To further explore how EEL-1 functions, here we performed affinity purification proteomics using Caenorhabditis elegans and identified the O-GlcNAc transferase OGT-1 as an EEL-1 binding protein. This observation was intriguing, as we know little about how OGT-1 affects neuron function. Using C. elegans biochemistry, we confirmed that the OGT-1/EEL-1 complex forms in neurons in vivo and showed that the human orthologs, OGT and HUWE1, also bind in cell culture. We observed that, like EEL-1, OGT-1 is expressed in GABAergic motor neurons, localizes to GABAergic presynaptic terminals, and functions cell-autonomously to regulate GABA neuron function. Results with catalytically inactive point mutants indicated that OGT-1 glycosyltransferase activity is dispensable for GABA neuron function. Consistent with OGT-1 and EEL-1 forming a complex, genetic results using automated, behavioral pharmacology assays showed that ogt-1 and eel-1 act in parallel to regulate GABA neuron function. These findings demonstrate that OGT-1 and EEL-1 form a conserved signaling complex and function together to affect GABA neuron function. |
| Databáze: | OpenAIRE |
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