High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia

Autor: Eduardo Colombari, Debora S. A. Colombari, Daniel B. Zoccal, Regina Célia Vendramini, José Vanderlei Menani, Guilherme Fleury Fina Speretta, Eduardo V. Lemes, Mirian Bassi
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade Federal de Santa Catarina (UFSC)
Rok vydání: 2018
Předmět:
Male
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Mean arterial pressure
Biometry
Physiology
Diaphragm
Chemoreflex
Blood Pressure
030204 cardiovascular system & hematology
Baroreflex
Diet
High-Fat
Statistics
Nonparametric
Article
Hypercapnia
03 medical and health sciences
Oxygen Consumption
0302 clinical medicine
Respiratory Rate
Heart Rate
Internal medicine
Heart rate
medicine
Animals
Hypoxia
Tidal volume
Abdominal Muscles
Diaphragm motor activity
Electromyography
business.industry
Respiration
General Neuroscience
digestive
oral
and skin physiology
Hypoxia (medical)
Rats
Diaphragm (structural system)
Plethysmography
Sympathetic modulation
Disease Models
Animal
Blood pressure
Exhalation
Cardiology
medicine.symptom
Pulmonary Ventilation
business
030217 neurology & neurosurgery
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1569-9048
Popis: Made available in DSpace on 2019-10-06T16:00:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Breathing disorders are commonly observed in association with obesity. Here we tested whether high-fat diet (HFD) impairs the chemoreflex ventilatory response. Male Holtzman rats (300–320 g) were fed with standard chow diet (SD) or HFD for 12 weeks. Then, tidal volume (V T ), respiratory frequency (f R ) and pulmonary ventilation (V E ) were determined in conscious rats during basal condition, hypercapnia (7% or 10% CO 2 ) or hypoxia (7% O 2 ). The mean arterial pressure (MAP), heart rate (HR) and baroreflex sensitivity were also evaluated in conscious rats. A group of anesthetized rats was used for the measurements of the activity of inspiratory (diaphragm) and expiratory (abdominal) muscles under the same gas conditions. Baseline f R , V T and V E were similar between SD and HFD rats. During hypercapnia, the increase of f R was exacerbated in conscious HFD rats (60 ± 3, vs. SD: 47 ± 3 Δ breaths.min −1 , P < 0.05). In anesthetized rats, hypercapnia strongly increased abdominal muscle activity in HFD group (238 ± 27, vs. basal condition: 100 ± 0.3%; P < 0.05), without significant change in SD group (129 ± 2.1, vs. basal condition: 100 ± 0.8%; P = 0.34). The ventilatory responses to hypoxia were similar between groups. In conscious HFD rats, MAP and HR were elevated and the baroreflex function was impaired (P < 0.05). These data demonstrated that 12 weeks of HFD exaggerate the ventilatory response activated by hypercapnia. The mechanisms involved in these responses need more investigation in future studies. Department of Physiology and Pathology School of Dentistry São Paulo State University (UNESP) Department of Clinical Analysis School of Pharmaceutical Sciences UNESP Department of Physiological Sciences Biological Sciences Center Federal University of Santa Catarina (UFSC), Rua Roberto Sampaio Gonzaga s/n, Trindade Department of Physiology and Pathology School of Dentistry São Paulo State University (UNESP) Department of Clinical Analysis School of Pharmaceutical Sciences UNESP FAPESP: 2013/13118-0 FAPESP: 2013/14850-6 FAPESP: 2013/17251-6 CAPES: 20131972 FAPESP: 2015/234677 CNPq: 304873/2014-4 CNPq: 310331/2017-0 CNPq: 425586/2016-2
Databáze: OpenAIRE
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