Modulating antioxidant activity and the controlled release capability of laccase mediated catechin grafting of chitosan
| Autor: | Katherinne Isabel Requejo, Suyeon Kim, Artur Cavaco-Paulo, Fernando G. Torres, Javier Nakamatsu, Karen N. Gonzales |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Bioengineering
02 engineering and technology 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Chitosan chemistry.chemical_compound Antioxidant activity o-quinones Organic chemistry Controlled release Laccase Science & Technology biology Chemistry Enzymatic grafting Coomassie Brilliant Blue Catechin 021001 nanoscience & nanotechnology biology.organism_classification Grafting 3. Good health 0104 chemical sciences 0210 nano-technology Methylene blue Myceliophthora thermophila Nuclear chemistry |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Laccase from ascomycete Myceliophthora thermophila was used for the oxidation and grafting of catechin (CA) and chitosan (CS). The enzymatic grafting of CA into CS resulted in the improvement of natural properties of chitosan films. The successful grafting of CA to CS was supported by UV-Visible and Fourier transform infrared (FT-IR) spectroscopies. The differences in the molecular weight and the degree of deacetylation of the CS affected on the enzymatic grafting rate and thus on the film properties like swelling behaviour, erosion rate, thermal property, tensile strength and breaking strain. The CS films prepared by enzymatic grafting presented high antioxidant activity compared to native CS films. The controlled release profiles of methylene blue and coomassie brilliant blue dye, having different acidity/basicity properties, loaded films were also studied with different release medium. The types of molecule loaded into films, the preparation manner of films and the release medium were decisive parameters affecting the release rate. This research was carried out by the Research Group of MaterialsModification at Pontificia Universidad Católica del Perú PUCP. Theauthors would like to acknowledge to the support of Dirección deGestión de la Investigación (DGI) and the Chemistry Departmentat Pontificia Universidad Católica del Perú PUCP for instrumentalanalysis. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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