| Autor: |
Kai Xia, Fulin Wang, Xingqiang Lai, Lin Dong, Peng Luo, Suyuan Zhang, Cuifeng Yang, Hong Chen, Yuanchen Ma, Weijun Huang, Wangsheng Ou, Yuyan Li, Xin Feng, Bin Yang, Congyuan Liu, Zhenmin Lei, Xiang’an Tu, Qiong Ke, Frank Fuxiang Mao, Chunhua Deng, Andy Peng Xiang |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Cell reports. Medicine. 3(11) |
| ISSN: |
2666-3791 |
| Popis: |
Leydig cell failure (LCF) caused by gene mutation results in testosterone deficiency and infertility. Serum testosterone levels can be recovered via testosterone replacement; however, established therapies have shown limited success in restoring fertility. Here, we use a luteinizing hormone/choriogonadotrophin receptor (Lhcgr)-deficient mouse model of LCF to investigate the feasibility of gene therapy for restoring testosterone production and fertility. We screen several adeno-associated virus (AAV) serotypes and identify AAV8 as an efficient vector to drive exogenous Lhcgr expression in progenitor Leydig cells through interstitial injection. We observe considerable testosterone recovery and Leydig cell maturation after AAV8-Lhcgr treatment in pubertal Lhcgr |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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