Estimation of relative glomerular capillary surface area in normal and hypertrophic rat kidneys
| Autor: | David W. Knutson, Frank Chieu, Cleaves M. Bennett, Richard J. Glassock, Bennett S. Kayser |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Microgram Kidney Glomerulus Renal function Kidney Nephrectomy Basement Membrane Muscle hypertrophy Internal medicine medicine Animals Autoantibodies Basement membrane Chemistry Body Weight Hypertrophy Organ Size Glomerular capillary Capillaries Rats medicine.anatomical_structure Endocrinology Nephrology Renal physiology Immunologic Techniques Binding Sites Antibody Rabbits Glomerular Filtration Rate |
| Zdroj: | Kidney international. 14(5) |
| ISSN: | 0085-2538 |
| Popis: | Estimation of relative glomerular capillary surface area in normal and hypertrophic rat kidneys. The quantity of antiglomerular basement membrane antibodies (antiGBM) binding to the glomeruli of rats 4 hr after i.v. injection of 660 µg of antiGMB was used as a measure of relative glomerular capillary surface area (Sr). Intact immature and adult rats (N = 27) weighing 46 to 440 g were studied to assess the effect of normal growth on Sr. Young adult rats (N = 36) were studied at 0, 8, 15, and 22 days following uninephrectomy or sham operation to assess the effect of hypertrophic kidney growth on Sr. Bound antiGBM increased from 95 µg to approximately 350 µg as rats grew from 46 to 200 g; further growth was associated with no further increases in bound antiGBM. In contrast, there was no progressive increase in Sr following uninephrectomy or sham operation despite a 45% increase in kidney weight at 22 days over the comparable kidney in the sham-operated rats (1.32 ± SEM 0.06 g vs. 0.91 ± SEM 0.01 g, P < 0.0001). Thus, increases in GFR during early normal kidney growth parallel anatomic increases in Sr, but increases in GFR with later growth or with compensatory hypertrophy in young adult rats are not accompanied by changes in Sr. |
| Databáze: | OpenAIRE |
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