Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection
| Autor: | Osman Safa Terzi, Aycan Nuriye Gazyagci, Tugce Sumer, Hasan Tarik Atmaca, Gungor Cagdas Dincel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Toxoplasma gondii Acute phase proteins Acute Lesion 03 medical and health sciences 0302 clinical medicine parasitic diseases medicine Serum amyloid A Chronic lcsh:QH301-705.5 lcsh:R5-920 biology business.industry Hemopexin medicine.disease biology.organism_classification Toxoplasmosis Macroglobulin Mononuclear cell infiltration 030104 developmental biology lcsh:Biology (General) Encephalitis Histopathology medicine.symptom business lcsh:Medicine (General) 030217 neurology & neurosurgery |
| Zdroj: | Laboratory Animal Research, Vol 35, Iss 1, Pp 1-9 (2019) |
| ISSN: | 2233-7660 |
| Popis: | Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, which occurs worldwide in mammals and birds. Brain is the primary target organ because Toxoplasma gondii is a ubiquitous intracellular parasite that causes most frequently life-threatening encephalitis in immunocompromised patients. Relation of tissue cysts number, histopathology score and acute phase proteins were investigated. In this study, 36 mice are infected with Me49 strain of Toxoplasma gondii. The control group has 6 healthy mice. After inoculation of Toxoplasma gondii, at 10., 15., 20., 30., 45., 60. days, 6 each mice euthanized after collection of blood samples. Hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin levels are determined by ELISA. Then, brain tissues were investigated histopathologically and lesions were scored. The average cyst numbers were determined by counting three samples (25 μl each) of each brain homogenate under light microscopy. Inflammatory reaction was observed on day 10 days after inoculation (d.a.i.) The lesions were characterized by perivascular mononuclear cell infiltration, focal mononuclear cell infiltration in the meninges, and glial proliferation. Tissue cysts were observed in all Toxoplasma gondii-infected groups. The highest lesion score was observed at 60 d.a.i. And the most tissue cyst number were on day 30. d.a.i. Serum levels of hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin were significantly higher than the control group on day 10–20., 10., 10–30., 10.,10–45 d.a.i., respectively. High level of acute phase proteins in mice on certain days infected with Toxoplasma gondii was exhibited a relationship between brain lesions and tissue cysts. |
| Databáze: | OpenAIRE |
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