An improved method for the enzymatic transformation of nucleosides into 5?-monophosphates
| Autor: | Sergei V. Kvach, A. I. Zinchenko, Vladimir N. Barai, Igor A. Mikhailopulo |
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| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Precipitation (chemistry) Chemistry Stereochemistry Erwinia herbicola Phosphotransferases food and beverages Nucleosides Bioengineering Improved method General Medicine Applied Microbiology and Biotechnology Combinatorial chemistry Phosphates Enzyme Activation Zinc Transformation (genetics) Enzyme Solubility Nucleoside phosphotransferase Erwinia Nucleoside Biotechnology |
| Zdroj: | Biotechnology Letters. 26:1847-1850 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-004-5311-4 |
| Popis: | An improved method to transform nucleosides into 5'-monophosphates using nucleoside phosphotransferase from Erwinia herbicola is reported. The method is based on the shift in the equilibrium state of the reaction to the formation of desired product due to its precipitation by Zn2+. Under optimal conditions, the extent of nucleoside transformations into nucleoside-5'-monophosphates were 41-91% (mol). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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