Liquid chromatography tandem mass spectrometry method for the quantitation of NTBC (2-(nitro-4-trifluoromethylbenzoyl)1,3-cyclohexanedione) in plasma of tyrosinemia type 1 patients

Autor: Maurice D. Laryea, Ute Spiekerkötter, Marc Lamshöft, Diran Herebian, Eva Thimm, Ertan Mayatepek
Rok vydání: 2009
Předmět:
Zdroj: Journal of Chromatography B. 877:1453-1459
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2009.03.014
Popis: In this study, we describe a bioanalytical method for quantification of NTBC in plasma of patients with hereditary tyrosinemia type 1 (HT-1) using high-performance liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). After protein precipitation with acetonitrile including Mesotrione as internal standard, separation of NTBC was achieved by RP-HPLC. Detection was performed by positive ion electrospray ionization (ESI) in selected reaction monitoring (SRM) mode. NTBC recovery in the developed method was found to be more than 90%. The lower limit of quantification was calculated to be 0.35 μM. The intra-day and inter-day precision of three different quality control samples (measured as RSD%) was less than 10% and 15%, respectively. The standard calibration curves showed good linearity within the range of 2.5–40 μM and the determined correlation coefficients were r 2 ≥ 0.995. This presented method is rapid, sensitive, specific and suitable for clinical practice and research.
Databáze: OpenAIRE
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