Effects of rhBMP-2/7 Heterodimer and RADA16 Hydrogel Scaffold on Bone Formation During Rabbit Mandibular Distraction
| Autor: | Yong-qing Tong, Song-yao Jiang, Geng-sheng Shi, Ling-fei Ren, Feng Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Bone Regeneration Bone density Bone Morphogenetic Protein 7 medicine.medical_treatment Osteogenesis Distraction Bone Morphogenetic Protein 2 Dentistry Mandible Bone morphogenetic protein 2 Random Allocation 03 medical and health sciences 0302 clinical medicine Bone Density Osteogenesis Transforming Growth Factor beta Distraction Animals Humans Medicine Bone regeneration Tissue Scaffolds business.industry Hydrogels 030206 dentistry Hydrogel scaffold Recombinant Proteins Bone morphogenetic protein 7 030104 developmental biology Otorhinolaryngology Distraction osteogenesis Surgery Rabbits Oral Surgery Peptides business |
| Zdroj: | Journal of Oral and Maxillofacial Surgery. 76:1092.e1-1092.e10 |
| ISSN: | 0278-2391 |
| DOI: | 10.1016/j.joms.2018.01.013 |
| Popis: | The effects of a recombinant human bone morphogenetic protein-2/7 (rhBMP-2/7) heterodimer and a RADA16 (Ac-RADARADARADARADA-CONH2) hydrogel scaffold on bone formation during distraction osteogenesis were evaluated.Forty New Zealand white rabbits, which underwent mandibular lengthening, were randomly divided into 5 groups. One group served as the control group. The others received 2 μg of rhBMP-2 homodimer, 2 μg of rhBMP-2/7 heterodimer, 100 μL of RADA16, or 100 μL of RADA16 plus 2 μg of rhBMP-2/7 heterodimer in the mandibular distraction gap at the beginning of distraction. Fluorine-18-labeled fluoride positron emission tomography was used to assess osteogenesis both after distraction and at the end of consolidation. Dual-energy x-ray absorptiometry (DEXA) examination and bone histologic findings were also evaluated.At the end of distraction, the radioactivity concentration in the distracted area was significantly greater in the RADA16 plus rhBMP-2/7 heterodimer group than in the other groups (P .01). The differences among the other 4 groups were also statistically significant in the following order: rhBMP-2/7 heterodimer group greater than the rhBMP-2 homodimer group, which was greater than the RADA16 group (or control group; P .05). However, the radioactivity concentration of the RADA16 group was slightly greater than that of the control group with a nonsignificant difference (P .05). By the end of consolidation, the activity in the control group, RADA16 group, rhBMP-2 homodimer group, and rhBMP-2/7 heterodimer group had significantly diminished (P .05). However, the activity in the RADA16 plus rhBMP-2/7 heterodimer group remained at the same level (P .05). The DEXA results and bone histologic findings indicated that more callus regeneration was noted in the RADA16 plus rhBMP-2/7 heterodimer group than in any other group.The use of rhBMP-2/7 heterodimer and RADA16 hydrogel scaffold significantly promoted mandibular distraction osteogenesis. |
| Databáze: | OpenAIRE |
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