DNA damage effect of cyprodinil and thiacloprid in adult zebrafish gills
| Autor: | Meltem Özgöçmen, Gülçin Yavuz Türel, Yusuf Ayvaz, İsmail Şen, Dilek Aşcı Çelik, Eda Evgen Tülüceoğlu, Vehbi Atahan Toğay |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Gills
Gill Turkey DNA damage Health Toxicology and Mutagenesis Thiazines 010501 environmental sciences 01 natural sciences Neonicotinoids chemistry.chemical_compound Animals Humans Environmental Chemistry Ecotoxicology Zebrafish 0105 earth and related environmental sciences biology Chemistry Environmental research General Medicine Pesticide Thiacloprid biology.organism_classification Pollution Molecular biology Comet assay Pyrimidines Comet Assay DNA Damage |
| Popis: | Cyprodinil and thiacloprid are two of the most commonly used pesticides in Turkey. It is more likely to reach humans or animals due to their widespread use. This study aims to investigate whether there is a DNA damage risk due to cyprodinil and thiacloprid exposure. Zebrafish, which is used as a model organism in health and environmental research, and comet assay were chosen to demonstrate this damage. Ten zebrafish per group were exposed to 2 different concentrations for each pesticides (0.31 and 0.155 mg/L for cyprodinil and 1.64 and 0.82 mg/L for thiacloprid) for 21 days. After, gills were excised and comet assay was performed. Photos of an average of 50 cells per slide were taken and were analyzed with visual evaluation program. DNA damage was found to be increased in the 0.31 mg/L cyprodinil, 0.82 mg/L thiacloprid, and 1.64 mg/L thiacloprid treatment groups when compared to the control group (p < 0.001). Average tail DNA percentage parameter values were 9.45 +/- 0.51, 10.30 +/- 0.34, 11.17 +/- 0.33, and 2.47 +/- 0.06 respectively. Cyprodinil and thiacloprid were identified as genotoxic agents that should be investigated further. |
| Databáze: | OpenAIRE |
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