Western Blotting Using Recombinant Hantaan Virus Nucleocapsid Protein Expressed in Silkworm as a Serological Confirmation of Hantavirus Infection in Human Sera
| Autor: | K, Yoshimatsu, J, Arikawa, H, Li, H, Kariwa, N, Hashimoto, N, Suzuki |
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| Rok vydání: | 1996 |
| Předmět: |
Hantavirus Infections
viruses Blotting Western Biology Transfection Sensitivity and Specificity Serology law.invention Capsid Antigen Reference Values law Chlorocebus aethiops parasitic diseases Nephropathia epidemica medicine Animals Humans Serologic Tests Vero Cells Hantaan virus Hantavirus General Veterinary Viral Core Proteins virus diseases Bombyx medicine.disease Virology Recombinant Proteins Staining Hemorrhagic Fever with Renal Syndrome Recombinant DNA Hantavirus Infection |
| Zdroj: | Journal of Veterinary Medical Science. 58:71-74 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.58.71 |
| Popis: | Recombinant Hantaan virus nucleocapsid protein expressed in silkworm larvae was applied as a serological diagnostic antigen in Western blots (WB) of human sera. The sensitivity of this method was similar to that of the IFA test. Hemorrhagic fever with renal syndrome (HFRS) and nephropathia epidemica diagnosed by their cross-reactivity in WB. The specificity of this method was higher than that of IFA test because the background was low. Sera that exhibited high background staining in the IFA test were readily diagnosed with this method. We recommended WB using recombinant Hantaan virus nucleocapsid antigen as a confirmatory procedure for the serodiagnosis of hantavirus. |
| Databáze: | OpenAIRE |
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