Expression and localization of alpha-tubulin N-acetyltransferase 1 in the reproductive system of male mice
| Autor: | Kenshiro Hara, Kentaro Tanemura, Kohei Umezu, Yudai Yamashita, Rin Yanai, Yuuki Hiradate |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system Alpha-tubulin N-acetyltransferase 1 (ATAT1) Spermiogenesis Genitalia Male Biology Male infertility Mice 03 medical and health sciences 0302 clinical medicine Acetyltransferases Microtubule Capacitation Testis medicine Animals Tissue Distribution Reproductive system Spermatogenesis Infertility Male reproductive and urinary physiology 030304 developmental biology Epididymis 0303 health sciences 030219 obstetrics & reproductive medicine urogenital system medicine.disease Spermatozoa Cell biology Mice Inbred C57BL medicine.anatomical_structure Seminiferous tubule Microtubule Proteins Original Article Animal Science and Zoology |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.2020-110 |
| Popis: | The structure of microtubules is essential for the fertilizing ability of spermatozoa. Acetylation of α-tubulin plays an important role in flagellar elongation and spermatozoa motility. Previous reports have suggested that alpha-tubulin N-acetyltransferase 1 (ATAT1) is the main acetyltransferase involved in the acetylation of α-tubulin. Although ATAT1 is reported to express in the testis, no information is available regarding its expression in elongated spermatids, epididymis, and mature spermatozoa. Hence, it remains unclear whether ATAT1 is involved in spermatozoa maturation and capacitation. Therefore, we evaluated the expression of ATAT1 in the mouse male reproductive system using immunostaining and western blotting. Our results showed that ATAT1 was expressed in spermatids during spermiogenesis in mouse testes, but its expression varied according to the seminiferous tubule stage. We observed ATAT1 in the cytoplasm of round spermatids, the flagella of elongated spermatids, and in the cytoplasm of step 16 spermatids, just before its release into the lumen. In addition, ATAT1 was expressed in epithelial cells of the epididymis. In spermatozoa of the cauda epididymis, ATAT1 expression was primarily observed in the midpiece of the spermatozoa. The localization of ATAT1 protein in the male germline was observed during spermiogenesis as well as during spermatozoa maturation. Our results suggest that ATAT1 may be involved in the formation of flagella and in the acetylation process, which has attracted attention in recent years regarding male infertility. |
| Databáze: | OpenAIRE |
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