Utility of pVHL, maspin, IMP3, S100P and Ki67 in the distinction of autoimmune pancreatitis from pancreatic ductal adenocarcinoma
| Autor: | Michael Friberg Bruun Nielsen, Sönke Detlefsen, Günter Klöppel, Michael Bau Mortensen, Ole Haagen Nielsen, Gitte Hedegaard Jensen |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Pathology medicine.medical_specialty Pancreatic ductal adenocarcinoma Scoring system Autoimmune Pancreatitis Pathology and Forensic Medicine Diagnosis Differential 03 medical and health sciences 0302 clinical medicine Ribonucleoproteins Small Nucleolar Metaplasia medicine Biomarkers Tumor Humans Serpins Autoimmune pancreatitis Aged business.industry Calcium-Binding Proteins Maspin Cell Biology Middle Aged medicine.disease Neoplasm Proteins ddc Pancreatic Neoplasms 030104 developmental biology Ductal Epithelium Ki-67 Antigen Von Hippel-Lindau Tumor Suppressor Protein 030220 oncology & carcinogenesis Normal pancreas Immunohistochemistry Female medicine.symptom business Carcinoma Pancreatic Ductal |
| Zdroj: | Hedegaard Jensen, G, Mortensen, M B, Klöppel, G, Nielsen, M F B, Nielsen, O & Detlefsen, S 2020, ' Utility of pVHL, maspin, IMP3, S100P and Ki67 in the distinction of autoimmune pancreatitis from pancreatic ductal adenocarcinoma ', Pathology, Research and Practice, vol. 216, no. 5, 152925 . https://doi.org/10.1016/j.prp.2020.152925 |
| DOI: | 10.1016/j.prp.2020.152925 |
| Popis: | Morphology plays an important role in the distinction of autoimmune pancreatitis (AIP) from pancreatic ductal adenocarcinoma (PDAC). However, we aimed to determine the utility of immunohistochemical tumor markers to contribute in the distinction of these entities. In surgical specimens with AIP (n = 20), PDAC (n = 20) and normal pancreas (n = 20), the expression of pVHL, maspin, IMP3, S100P and Ki67 was examined. We evaluated intralobular reactive ducts / acinoductal metaplasia (ILDs) and extralobular ducts (ELDs) in AIP, neoplastic glands in PDAC, and ductal epithelium in the normal pancreas, using a five-tiered scoring system. The Ki67 hot spot index (Ki67-HSPI) was determined manually and using automated digital imaging analysis of virtual double stains of Ki67 and CK8. Besides, sequential dual-immunohistochemical staining of maspin/pVHL, maspin/IMP3 and Ki67/maspin was performed in a subset of the specimens. Strong overexpression of IMP3, maspin, S100P and Ki67 and loss of pVHL was observed in PDAC compared to AIP and normal pancreas. In AIP however, focal and weak aberrant expression was observed with the following proportions in ILDs/ELDs: pVHL in 45 %/85 %, maspin in 30 %/70 %, IMP3 in 55 %/5%, S100P in 10 %/35 % and Ki67-HSPI >20 % in 15 %/70 %. At least two markers were aberrantly expressed in ILDs/ELDs in 45 %/60 %. The aberrant expression was more pronounced in type 2 AIP compared to type 1. In conclusion, our data indicate that pVHL, maspin, IMP3, S100P and Ki67 can be focal and weak aberrantly expressed in AIP. However, when used as a panel, these markers seem to be useful for the differentiation of AIP from PC. |
| Databáze: | OpenAIRE |
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