Biochemical characterization of crystallins from pigeon lenses: structural and sequence analysis of pigeon δ-crystallin
| Autor: | Shyh-Horng Chiou, Chin-Chun Hung, Chou-Wen Lin |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Sequence analysis
Protein subunit Molecular Sequence Data Size-exclusion chromatography Biophysics Biology Polymerase Chain Reaction Biochemistry Species Specificity Structural Biology Crystallin Complementary DNA Geese Lens Crystalline Animals Amino Acid Sequence Amino Acids Columbidae Molecular Biology Gel electrophoresis Base Sequence Crystallins Molecular biology Argininosuccinate lyase eye diseases Ducks Isoelectric point sense organs Chickens Glycogen |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1160:317-324 |
| ISSN: | 0167-4838 5286-5290 |
| DOI: | 10.1016/0167-4838(92)90094-t |
| Popis: | Crystallins from pigeon eye lenses were isolated and purified by gel-permeation chromatography and characterized by gel electrophoresis, amino-acid composition and sequence analysis. Alpha- and beta-crystallins could be obtained in relatively pure forms by single-step size-exclusion chromatography whereas an extra step of ion-exchange chromatography was needed for the separation of delta-crystallin from the beta-crystallin fraction. In contrast to most characterized vertebrate species, a large amount of glycogen is eluted as a high molecular form in the first peak of the gel filtration column. Pigeon delta-crystallin, similar to duck and reptilian delta-crystallins, exists as a tetrameric structure of about 200 kDa in the native form and is composed of one major subunit of 50 kDa with heterogeneous isoelectric points spreading in a range of 4.7 to 6.8. In contrast to those obtained from duck, goose and caiman, delta-crystallin isolated from the pigeon lens possessed very little argininosuccinate lyase activity. However, pigeon delta-crystallin can still cross-react with the antibody against enzymically active duck delta-crystallin as revealed by the sensitive immunoblotting technique. It was also shown that the delta-crystallin content of the total pigeon soluble proteins decreased with the age of the animal. Structural analysis of purified delta-crystallin fraction was made with respect to its amino-acid composition and protein primary sequence. N-terminal sequence analysis indicated the presence of blocked amino-termini in all crystallin fractions of pigeon lenses. Therefore, a sequence analysis of PCR (polymerase chain reaction) amplified delta-crystallin cDNA was employed to deduce the protein sequence of this crystallin. Structural comparison of delta-crystallin sequences from pigeon, chicken and duck lenses casts some doubts on the recent claim that His-89--Gln mutation in the chicken delta-crystallin may account for the loss of argininosuccinate lyase activity in this avian species, as compared to high enzymic activity in the duck crystallin (Barbosa et al. (1991) J. Biol. Chem. 266, 5286-5290). |
| Databáze: | OpenAIRE |
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