DNA-based communication in populations of synthetic protocells
| Autor: | B. V. V. S. Pavan Kumar, Shuo Yang, Stephen Mann, Andrew Phillips, Ardjan J. van der Linden, Tom F. A. de Greef, Neil Dalchau, Bas W. A. Bögels, Pascal A. Pieters, Alex Joesaar |
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| Přispěvatelé: | Chemical Biology, Computational Biology |
| Jazyk: | angličtina |
| Předmět: |
Protocell
Computer science Process (engineering) Logic Distributed computing Biomedical Engineering Bioengineering BrisSynBio 02 engineering and technology Cell Communication 010402 general chemistry 01 natural sciences Article law.invention Synthetic biology chemistry.chemical_compound DNA computing law DNA/metabolism General Materials Science Electrical and Electronic Engineering Modularity (networks) Molecular communication Bristol BioDesign Institute DNA Artificial Cells/cytology 021001 nanoscience & nanotechnology Condensed Matter Physics Atomic and Molecular Physics and Optics 0104 chemical sciences SYNTHETIC BIOLOGY chemistry Synthetic Biology/methods Scalability Artificial Cells Synthetic Biology Biophysical Chemistry 0210 nano-technology Signal Transduction |
| Zdroj: | Nature Nanotechnology, 14(4), 369-378. Nature Publishing Group Joesaar, A, Yang, S, Bögels, B, van der Linden, A, Pieters, P, Kumar, B V V S P, Dalchau, N, Phillips, A, Mann, S & de Greef, T F A 2019, ' DNA-based communication in populations of synthetic protocells ', Nature Nanotechnology, vol. 14, no. 4, pp. 369-378 . https://doi.org/10.1038/s41565-019-0399-9 Nature Nanotechnology Nature nanotechnology Nature Nanotechnology, 14, 4, pp. 369 Nature Nanotechnology, 14, 369 |
| ISSN: | 1748-3395 1748-3387 |
| DOI: | 10.1038/s41565-019-0399-9 |
| Popis: | Developing molecular communication platforms based on orthogonal communication channels is a crucial step towards engineering artificial multicellular systems. Here, we present a general and scalable platform entitled ‘biomolecular implementation of protocellular communication’ (BIO-PC) to engineer distributed multichannel molecular communication between populations of non-lipid semipermeable microcapsules. Our method leverages the modularity and scalability of enzyme-free DNA strand-displacement circuits to develop protocellular consortia that can sense, process and respond to DNA-based messages. We engineer a rich variety of biochemical communication devices capable of cascaded amplification, bidirectional communication and distributed computational operations. Encapsulating DNA strand-displacement circuits further allows their use in concentrated serum where non-compartmentalized DNA circuits cannot operate. BIO-PC enables reliable execution of distributed DNA-based molecular programs in biologically relevant environments and opens new directions in DNA computing and minimal cell technology. Semipermeable proteinosome membranes allow complex DNA message communication through compartmentalization and protect the DNA circuits from degradation in a biological environment. |
| Databáze: | OpenAIRE |
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