Therapeutic effect of human umbilical cord blood mesenchymal stem cells combined with G-CSF on rats with acute liver failure
| Autor: | Haiou Chen, Jinmao Liao, Yihe Lin, Meng Liu, Shigang Tang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Biophysics Inflammation Apoptosis Mesenchymal Stem Cell Transplantation Biochemistry Umbilical cord Andrology Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Granulocyte Colony-Stimulating Factor Medicine Animals Humans Molecular Biology Cell Proliferation Liver injury TUNEL assay business.industry Liver cell Multipotent Stem Cells Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Liver Failure Acute medicine.disease Fetal Blood Granulocyte colony-stimulating factor Oxidative Stress 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Liver function medicine.symptom business |
| Zdroj: | Biochemical and biophysical research communications. 517(4) |
| ISSN: | 1090-2104 |
| Popis: | Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) have been used to facilitate healing in animal models of liver injury, while granulocyte colony-stimulating factor (G-CSF) has been shown to stimulate stem cell mobilization and these cells may contribute to liver repair. hUCB-MSCs were characterized by flow cytometry, and transplanted into rats with d-galactosamine (D-GalN)/lipopolysaccharides (LPS)-induced acute liver failure (ALF) together with granulocyte colony-stimulating factor (G-CSF). Liver function, oxidative stress and pro-inflammatory cytokines expressions were examined using enzyme-linked immunosorbent assay (ELISA). Hematoxylin-eosin (HE) staining was used to observe the morphological changes. Apoptosis was investigated by terminal dUTP nick end labeling (TUNEL) staining. Bromodeoxyuridine (BrdU) cell proliferation assay was analyzed by immunofluorescence and immunohistochemistry. In the results, cultured hUCB-MSCs displayed proliferation and adipogenic and osteogenic differentiation potentials. hUCB-MSCs in combination with G-CSF significantly attenuated ALF-induced liver function injury. Furthermore, hUCB-MSCs and G-CSF treatment remarkably suppressed the secretions of pro-inflammatory cytokines and MDA activation induced by ALF. In addition, inflammation, lesions and cell apoptosis in liver tissues were obviously ameliorated by application of hUCB-MSCs and G-CSF. In conclusion, hUCB-MSCs, alone or co-treatment with G-CSF could ameliorate ALF in rats by inhibiting liver function injury, production of pro-inflammatory cytokines, oxidative stress, and liver cell apoptosis. |
| Databáze: | OpenAIRE |
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