Formation of secretory vesicles in permeabilized cells: a salt extract from yeast membranes promotes budding of nascent secretory vesicles from the trans-Golgi network of endocrine cells
| Autor: | Wai Lam W. Ling, Dennis Shields |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Cell Membrane Permeability
Saccharomyces cerevisiae Golgi Apparatus Pituitary neoplasm Biology Cytoplasmic Granules Transfection Biochemistry Cell Line Cell membrane symbols.namesake Cytosol medicine Animals Humans Pituitary Neoplasms Protein Precursors Molecular Biology Mammals Tissue Extracts Vesicle Cell Membrane Brain Cell Biology Golgi apparatus biology.organism_classification Secretory Vesicle Yeast Recombinant Proteins Cell biology Rats Secretory protein medicine.anatomical_structure Pituitary Gland symbols Cattle Somatostatin Research Article |
| Popis: | The mechanism of secretory-vesicle formation from the trans-Golgi network (TGN) of endocrine cells is poorly understood. To identify cytosolic activities that facilitate the formation and fission of nascent secretory vesicles, we treated permeabilized pituitary GH3 cells with high salt to remove endogenous budding factors. Using this cell preparation, secretory-vesicle budding from the TGN required addition of exogenous cytosol and energy. Mammalian cytosols (GH3 cells and bovine brain) promoted post-TGN vesicle formation. Most significantly, a salt extract of membranes from the yeast Saccharomyces cerevisiae, a cell lacking a regulated secretory pathway, stimulated secretory vesicle budding in the absence of mammalian cytosolic factors. These results demonstrate that the factors which promote secretory-vesicle release from the TGN are conserved between yeast and mammalian cells. |
| Databáze: | OpenAIRE |
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