Dietary supplementation with strawberry induces marked changes in the composition and functional potential of the gut microbiome in diabetic mice
| Autor: | Chrissa Petersen, Umesh D. Wankhade, Sree V. Chintapalli, J. David Symons, Thunder Jalili, Kiana Wong, Kartik Shankar, Jennifer Ellen Mueller, Pon Velayutham Anandh Babu, Brian D. Piccolo, Zhenquan Jia, Divya Bharat |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Endocrinology Diabetes and Metabolism Clinical Biochemistry Gut flora Fragaria Biochemistry Article Diabetes Mellitus Experimental 03 medical and health sciences Microbial ecology Animals Food science Molecular Biology Bifidobacterium 030109 nutrition & dietetics Nutrition and Dietetics biology Verrucomicrobia Metabolism biology.organism_classification 16S ribosomal RNA Mice Mutant Strains Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology Dietary Supplements Receptors Leptin Composition (visual arts) Metabolic Networks and Pathways Bacteria |
| Zdroj: | The Journal of Nutritional Biochemistry. 66:63-69 |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2019.01.004 |
| Popis: | Gut microbiota contributes to the biological activities of berry anthocyanins by transforming them into bioactive metabolites, and anthocyanins support the growth of specific bacteria, indicating a two-way relationship between anthocyanins and microbiota. In the present study, we tested the hypothesis that strawberry supplementation alters gut microbial ecology in diabetic db/db mice. Control (db/+) and diabetic (db/db) mice (7 weeks old) consumed standard diet or diet supplemented with 2.35% freeze-dried strawberry (db/db?+?SB) for 10 weeks. Colon contents were used to isolate bacterial DNA. V4 variable region of 16S rRNA gene was amplified. Data analyses were performed using standardized pipelines (QIIME 1.9 and R packages). Differences in predictive metagenomics function were identified by PICRUSt. Principal coordinate analyses confirmed that the microbial composition was significantly influenced by both host genotype and strawberry consumption. Further, a-diversity indices and s-diversity were different at the phylum and genus levels, and genus and operational taxonomical units levels, respectively (P |
| Databáze: | OpenAIRE |
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