Role of CNP in human airways: cGMP-mediated stimulation of ciliary beat frequency
| Autor: | Cara Geary, R. C. Boucher, C. W. Davis, A. M. Paradiso |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Nitroprusside Pulmonary and Respiratory Medicine medicine.medical_specialty Time Factors Adolescent Physiology Movement 8-Bromo Cyclic Adenosine Monophosphate Stimulation Epithelium Adenosine Triphosphate Physiology (medical) Internal medicine medicine Humans Cilia Ciliary beating Cyclic GMP Cells Cultured Chemistry Isoproterenol Proteins Natriuretic Peptide C-Type Cell Biology Middle Aged Kinetics Nasal Mucosa Endocrinology Female Airway Atrial Natriuretic Factor |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 268:L1021-L1028 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.1995.268.6.l1021 |
| Popis: | Ciliated airway epithelial cells contribute to mucociliary transport systems via ciliary beating and electrolyte transport mechanisms. Both of these activities are regulated by agonists acting through intracellular calcium- and adenosine 3',5'-cyclic monophosphate (cAMP)-dependent processes (5, 15, 18, 27). This study examines the role of guanosine 3',5'-cyclic monophosphate (cGMP) in the regulation of both ciliary beat frequency (CBF) and electrolyte transport in human airway epithelia (HAE). In a previous report, cGMP production in HAE was observed after stimulation with either C-type natriuretic peptide (CNP) or sodium nitroprusside (SNP) (6). In this study, CNP was found to increase CBF by 30 +/- 6.9%, and this effect was mimicked by the cGMP analogue, 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), but not by sodium nitroprusside. CNP-induced increases in CBF do not appear to be mediated by changes in either intracellular calcium or cAMP levels. Using modified Ussing chambers, we also investigated CNP's potential modulation of sodium and chloride transport rates. Neither CNP, nor SNP, nor 8-BrcGMP altered active ion transport rates. We conclude that CNP regulates ciliary beat via cGMP-dependent mechanisms, whereas no effect of CNP or cGMP on ion transport was detected. |
| Databáze: | OpenAIRE |
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