Development of A Sensitive and Specific Epitope-Blocking ELISA for Universal Detection of Antibodies to Human Enterovirus 71 Strains

Autor: Qingfeng Chen, Vincent T. K. Chow, Yunrui Tan, Jimmy Kwang, Tanja K. Kiener, Fang He, Kattur Venkatachalam Ashok Raj, Xiao Fang Lim, Manli Tang
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Applied Microbiology
lcsh:Medicine
Antibodies
Viral
Epitope
Immunoglobulin G
Serology
Epitopes
Seroepidemiologic Studies
Emerging Viral Diseases
lcsh:Science
Child
Multidisciplinary
Child Health
Antibodies
Monoclonal
Infectious Diseases
Capsid
Medical Microbiology
Child
Preschool
Medicine
Public Health
Antibody
Research Article
Biotechnology
Infectious Disease Control
Adolescent
medicine.drug_class
Immunology
Guinea Pigs
Enzyme-Linked Immunosorbent Assay
Biology
Coxsackievirus
Monoclonal antibody
Microbiology
Sensitivity and Specificity
Virus
Diagnostic Medicine
Neutralization Tests
Virology
medicine
Enterovirus Infections
Animals
Humans
Microbial Pathogens
lcsh:R
Immunity
Infant
biology.organism_classification
Antibodies
Neutralizing
Enterovirus A
Human
Viral Disease Diagnosis
Emerging Infectious Diseases
biology.protein
lcsh:Q
Clinical Immunology
Capsid Proteins
Viral Transmission and Infection
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 1, p e55517 (2013)
ISSN: 1932-6203
Popis: Background Human Enterovirus 71 (EV71) is a common cause of hand, foot and mouth disease (HFMD) in young children. It is often associated with severe neurological diseases and mortalities in recent outbreaks across the Asia Pacific region. Currently, there is no efficient universal antibody test available to detect EV71 infections. Methodology/Principal Finding In the present study, an epitope-blocking ELISA was developed to detect specific antibodies to human EV71 viruses in human or animal sera. The assay relies on a novel monoclonal antibody (Mab 1C6) that specifically binds to capsid proteins in whole EV71 viruses without any cross reaction to any EV71 capsid protein expressed alone. The sensitivity and specificity of the epitope-blocking ELISA for EV71 was evaluated and compared to microneutralization using immunized animal sera to multiple virus genotypes of EV71 and coxsackieviruses. Further, 200 serum sample from human individuals who were potentially infected with EV71 viruses were tested in both the blocking ELISA and microneutralization. Results indicated that antibodies to EV71 were readily detected in immunized animals or human sera by the epitope blocking ELISA whereas specimens with antibodies to other enteroviruses yielded negative results. This assay is not only simpler to perform but also shows higher sensitivity and specificity as compared to microneutralization. Conclusion The epitope-blocking ELISA based on a unique Mab 1C6 provided highly sensitive and 100% specific detection of antibodies to human EV71 viruses in human sera.
Databáze: OpenAIRE
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