Post-mortem molecular investigations of SARS-CoV-2 in an unexpected death of a recent kidney transplant recipient
| Autor: | Daniel A. Muruve, Emily Lauren Simms, Dylan R. Pillai, Hyunjae Chung, Amy Bromley, Justin Chun, Lisa Oberding, Braedon McDonald |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
basic (laboratory) research/science
donors and donation: donor‐derived infections Pathology medicine.medical_specialty Emergency Use Authorization Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Case Report kidney transplantation/nephrology In situ hybridization 030230 surgery clinical research/practice kidney transplantation: living donor 03 medical and health sciences 0302 clinical medicine Pandemic patient safety medicine infection and infectious agents‐viral Immunology and Allergy Pharmacology (medical) Cause of death Transplantation Kidney business.industry body regions medicine.anatomical_structure Immunohistochemistry business pathology/histopathology |
| Zdroj: | American Journal of Transplantation |
| ISSN: | 1600-6135 |
| DOI: | 10.1111/ajt.16549 |
| Popis: | Solid organ transplant recipients are vulnerable to severe infection during induction therapy. We report a case of a 67‐year‐old male who died unexpectedly 10 days after receiving a kidney transplant on February 10, 2020. There was no clear cause of death, but COVID‐19 was considered retrospectively, as the death occurred shortly after the first confirmed case of COVID‐19 in Canada. We confirmed the presence of SARS‐CoV‐2 components in the renal allograft and native lung tissue using immunohistochemistry for SARS‐CoV‐2 spike protein and RNA scope in situ hybridization for SARS‐CoV‐2 RNA. Results were reaffirmed with the Food and Drug Administration Emergency Use Authorization approved Bio‐Rad SARS‐CoV‐2 digital droplet PCR for the kidney specimen. Our case highlights the importance of patient autopsies in an unfolding global pandemic and demonstrates the utility of molecular assays to diagnose SARS‐CoV‐2 post‐mortem. SARS‐CoV‐2 infection during induction therapy may portend a fatal clinical outcome. We also suggest COVID‐19 may be transmittable via renal transplant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text