MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development
| Autor: | Ioannis S. Vlachos, Georgia Kouroupi, Kanella Prodromidou, Artemis G. Hatzigeorgiou, Maria Gaitanou, Rebecca Matsas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Doublecortin Domain Proteins
Male 0301 basic medicine PAX6 Transcription Factor early neurons progenitor cell QH301-705.5 Neurogenesis Science Human Embryonic Stem Cells Biology Axonogenesis General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences 0302 clinical medicine Neural Stem Cells Neuroblast doublecortin Subplate medicine Humans Progenitor cell Biology (General) General Immunology and Microbiology Gene Expression Profiling General Neuroscience Neuropeptides Gene Expression Regulation Developmental General Medicine PAX6 MicroRNAs Corticogenesis 030104 developmental biology medicine.anatomical_structure neuritogenesis Medicine Microtubule-Associated Proteins Neural development Neuroscience 030217 neurology & neurosurgery Research Article Developmental Biology Human neural induction |
| Zdroj: | eLife, Vol 9 (2020) eLife |
| Popis: | Integrating differential RNA and miRNA expression during neuronal lineage induction of human embryonic stem cells we identified miR-934, a primate-specific miRNA that displays a stage-specific expression pattern during progenitor expansion and early neuron generation. We demonstrate the biological relevance of this finding by comparison with data from early to mid-gestation human cortical tissue. Further we find that miR-934 directly controls progenitor to neuroblast transition and impacts on neurite growth of newborn neurons. In agreement, miR-934 targets are involved in progenitor proliferation and neuronal differentiation whilst miR-934 inhibition results in profound global transcriptome changes associated with neurogenesis, axonogenesis, neuronal migration and neurotransmission. Interestingly, miR-934 inhibition affects the expression of genes associated with the subplate zone, a transient compartment most prominent in primates that emerges during early corticogenesis. Our data suggest that mir-934 is a novel regulator of early human neurogenesis with potential implications for a species-specific evolutionary role in brain function. |
| Databáze: | OpenAIRE |
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