Chemical priming of immunity without costs to plant growth

Autor: Pierre Pétriacq, Beining Chen, Matthew A. Sellwood, Roland E. Schwarzenbacher, Jurriaan Ton, Victor Flors, Estrella Luna, Will Buswell
Přispěvatelé: University of Sheffield [Sheffield], Universitat Jaume I
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Indoles
b-homoserine
Physiology
Arabidopsis
Plant Development
Plant Science
Plant disease resistance
01 natural sciences
induced resistance
Cell wall
03 medical and health sciences
chemistry.chemical_compound
Solanum lycopersicum
Protein Domains
b-aminobutyric acid (BABA)
Homoserine
Camalexin
Computer Simulation
Plant Immunity
priming
Psychological repression
IBI1
ComputingMilieux_MISCELLANEOUS
Disease Resistance
Plant Diseases
Botrytis cinerea
Hyaloperonospora arabidopsidis
biology
Arabidopsis Proteins
Aminobutyrates
Jasmonic acid
Fungi
food and beverages
Ethylenes
biology.organism_classification
Cell biology
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Thiazoles
030104 developmental biology
chemistry
Mutation
Salicylic Acid
Salicylic acid
Signal Transduction
010606 plant biology & botany
crop protection
Zdroj: New Phytologist
New Phytologist, Wiley, 2018, 218 (3), pp.1205-1216. ⟨10.1111/nph.15062⟩
Repositori Universitat Jaume I
Universitat Jaume I
ISSN: 0028-646X
1469-8137
DOI: 10.1111/nph.15062⟩
Popis: b-Aminobutyric acid (BABA) induces broad-spectrum disease resistance, but also represses plant growth, which has limited its exploitation in crop protection. BABA perception relies on binding to the aspartyl-tRNA synthetase (AspRS) IBI1, which primes the enzyme for secondary defense activity. This study aimed to identify structural BABA analogues that induce resistance without stunting plant growth. Using site-directed mutagenesis, we demonstrate that the (L)-aspartic acid-binding domain of IBI1 is critical for BABA perception. Based on interaction models of this domain, we screened a small library of structural BABA analogues for growth repression and induced resistance against biotrophic Hyaloperonospora arabidopsidis (Hpa). A range of resistance-inducing compounds were identified, of which (R)-b-homoserine (RBH) was the most effective. Surprisingly, RBH acted through different pathways than BABA. RBH-induced resistance (RBH-IR) against Hpa functioned independently of salicylic acid, partially relied on camalexin, and was associated with augmented cell wall defense. RBH-IR against necrotrophic Plectosphaerella cucumerina acted via priming of ethylene and jasmonic acid defenses. RBH-IR was also effective in tomato against Botrytis cinerea. Metabolic profiling revealed that RBH, unlike BABA, does not majorly affect plant metabolism. RBH primes distinct defense pathways against biotrophic and necrotrophic pathogens without stunting plant growth, signifying strong potential for exploitation in crop protection.
Databáze: OpenAIRE
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