Subcellular targeting of human interleukin-10 in plants
| Autor: | Rima Menassa, R. T. Rymerson, Wendy Kennette, A. M. Jevnikar, Vi Nguyen, Jim Brandle |
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| Rok vydání: | 2004 |
| Předmět: |
Chloroplasts
Cell Bioengineering Biology Mitochondrion Protein Engineering Applied Microbiology and Biotechnology Transit Peptide Tobacco medicine Humans food and beverages Gene targeting Biological activity General Medicine Protein engineering Plants Genetically Modified In vitro Recombinant Proteins Interleukin-10 Mitochondria Chloroplast Molecular Weight Plant Leaves medicine.anatomical_structure Biochemistry Gene Targeting Mutagenesis Site-Directed Biotechnology Subcellular Fractions |
| Zdroj: | Journal of biotechnology. 108(2) |
| ISSN: | 0168-1656 |
| Popis: | The utility of plants for the production of a wide range of recombinant proteins is now clearly established. However, the challenge remains to produce these proteins at sufficient concentrations for extraction to be economically feasible. In this paper, we have investigated the ability of plant cells to accumulate the human interleukin-10 (IL-10) protein targeted to chloroplasts and mitochondria. We found that IL-10 accumulates in chloroplasts only if a 6 x His tag is added at the C-terminus of the protein. The hexapeptide may provide protection from degradation. Conversely, the IL-10 protein does not accumulate in mitochondria. Analysis of the chloroplast-targeted IL-10 protein revealed only monomeric IL-10 and limited biological activity in in vitro cell assays. |
| Databáze: | OpenAIRE |
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