The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge
| Autor: | Kuldeep Verma, Sivaram V. S. Mylavarapu, Rajaiah Pergu, Kumari Pushpa, Harsh Kumar, Amrita Kumari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Embryo
Nonmammalian Cell division Endosome Endocytic cycle Green Fluorescent Proteins Vesicular Transport Proteins Exocyst complex Exocyst Polar Bodies Biology environment and public health ESCRT Chromosome segregation 03 medical and health sciences 0302 clinical medicine Animals Humans Cleavage furrow Caenorhabditis elegans Caenorhabditis elegans Proteins 030304 developmental biology Cytokinesis rab5 GTP-Binding Proteins 0303 health sciences Endosomal Sorting Complexes Required for Transport fungi Cell Membrane Cell Biology Endocytosis Cell biology enzymes and coenzymes (carbohydrates) Protein Subunits ESCRT III biological phenomena cell phenomena and immunity 030217 neurology & neurosurgery Rab GTPase Research Article HeLa Cells Protein Binding |
| Zdroj: | Journal of Cell Science |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | Cytokinesis is the final step of cell division following chromosome segregation that generates two daughter cells. The conserved exocyst complex is required for scission of the intercellular cytokinetic bridge, although the molecular mechanisms it employs in this process are unclear. We identify and validate the early endocytic GTPase Rab5 as interacting with the exocyst complex in mammalian cells. Rab5 localizes in the cytokinetic bridge and on the midbody ring in a manner similar to the exocyst complex. Depletion of Rab5 led to delayed abscission. Caenorhabditis elegans orthologs of both exocyst complex subunits and Rab5 localize along the cleavage furrow and are required for cytokinesis in early embryos. Cytokinetic cells depleted of either Rab5 or the exocyst subunits Exoc3 and Exoc4 showed impaired deposition of the endosomal sorting complexes required for transport (ESCRT) III subunits CHMP2B and/or CHMP4B near the midbody ring. The study reveals an evolutionarily conserved role for the early endocytic marker Rab5 in cytokinetic abscission. In addition, it uncovers a key requirement of the exocyst and Rab5 for the delivery of components of the membrane-severing ESCRT III machinery to complete cytokinesis. Summary: The conserved exocytic vesicle-tethering exocyst complex and the endocytic master regulator Rab5 mutually interact and are required to deliver the ESCRT III membrane scission apparatus for completion of cytokinesis. |
| Databáze: | OpenAIRE |
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