Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei
| Autor: | Fuli Zheng, Frank Voncken, Claudia Colasante |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Iron 030231 tropical medicine Genes Fungal Genes Protozoan Trypanosoma brucei brucei Protozoan Proteins Saccharomyces cerevisiae Biology Mitochondrion Trypanosoma brucei Mitochondrial Membrane Transport Proteins 03 medical and health sciences 0302 clinical medicine Iron-Binding Proteins parasitic diseases Protein biosynthesis Inner mitochondrial membrane Molecular Biology Cation Transport Proteins Mitochondrial transport Phylogeny 030304 developmental biology 0303 health sciences biology.organism_classification Mitochondrial carrier Yeast Mitochondria Complementation Biochemistry Parasitology |
| Zdroj: | Molecular and biochemical parasitology. 233 |
| ISSN: | 1872-9428 |
| Popis: | Similar to higher eukaryotes, the protist parasite T. brucei harbours several iron-containing proteins that regulate DNA and protein processing, oxidative stress defence and mitochondrial respiration. The synthesis of these proteins occurs either in the cytoplasm or within the mitochondrion. For mitochondrial iron cluster protein synthesis, iron needs to be transported across the solute impermeable mitochondrial membrane. In T. brucei we previously identified 24 mitochondrial carrier proteins (TbMCPs) sharing conserved structural and functional features with those from higher eukaryotes. One of these carriers (TbMCP17) displayed high similarity with the iron carriers MRS3, MRS4 from yeast and mitoferrin from mammals, insects and plants. In the present study we demonstrated that TbMCP17 functions as an iron carrier by complementation studies using MRS3/4-deficient yeast. Depletion of TbMCP17 in procyclic form T. brucei resulted in growth deficiency, increased sensitivity to iron deprivation, and lowered mitochondrial iron content. Taken together our results suggest that TbMCP17 functions as a mitochondrial iron transporter in the parasite T. brucei. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect