Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia

Autor:	Rafa Almeer, Latifa E. Qassim, Dina M. Metwally, Isra Al-Turaiki, Manal F. El-Khadragy
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Veterinary medicine lcsh:Medicine Artificial Gene Amplification and Extension Polymerase Chain Reaction Animal Diseases law.invention Database and Informatics Methods Camels law Genotype Medicine and Health Sciences Echinococcus granulosus lcsh:Science Phylogeny Polymerase chain reaction Data Management Mammals Multidisciplinary Eukaryota Agriculture Phylogenetic Analysis Ruminants 030108 mycology & parasitology Echinococcosis Phylogenetics Helminth Infections Vertebrates Livestock Sequence Analysis Research Article Neglected Tropical Diseases Multiple Alignment Calculation Computer and Information Sciences endocrine system Camelus Bioinformatics Sequence analysis Saudi Arabia Biology Research and Analysis Methods Electron Transport Complex IV 03 medical and health sciences Computational Techniques parasitic diseases Parasitic Diseases medicine Animals Cytochrome c oxidase Evolutionary Systematics Molecular Biology Techniques Molecular Biology Taxonomy Evolutionary Biology Sheep Base Sequence business.industry lcsh:R Organisms Biology and Life Sciences Sequence Analysis DNA Tropical Diseases medicine.disease biology.organism_classification Split-Decomposition Method Echinococcus Amniotes biology.protein lcsh:Q business Sequence Alignment
Zdroj:	PLoS ONE, Vol 13, Iss 4, p e0195016 (2018) PLoS ONE
ISSN:	1932-6203
Popis:	The diversity and importance of Echinococcus species in domesticated animals in Saudi Arabia are poorly understood. In this study, 108 singular (hydatid) cysts were collected from goats (n = 25), sheep (n = 56) and camels (n = 27). DNA was extracted from the protoscoleces of individual fertile cysts and used for polymerase chain reaction (PCR) amplification of mitochondrial subunit 1 of the cytochrome c oxidase 1 (cox1) gene. Amplicon sequencing results revealed the presence of Echinococcus granulosus sensustricto (s.s.) (genotypes G1-G3) in 16 of the17 sheep cysts and 2 of the 27 camel cysts.of these samples, 18 (2 camel and 16 sheep) were divided into genotypes G1, G2, and G3.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91bb074bbd8e65089c38d08c40a6eaf4 http://europepmc.org/articles/PMC5905957?pdf=render Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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