Adipose tissue-derived stem cell therapy for prevention and treatment of erectile dysfunction in a rat model of Peyronie's disease
| Autor: | A. Gokce, Gökçe, Ahmet, Z. Y. Abd Elmageed, G. F. Lasker, M. Bouljihad, H. Kim, L. W. Trost, P. J. Kadowitz, A. B. Abdel-Mageed, S. C. Sikka, W. J. Hellstrom |
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| Přispěvatelé: | Sakarya Üniversitesi/Tıp Fakültesi/Cerrahi Tıp Bilimleri Bölümü, Gökçe, Ahmet |
| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty Urology Endocrinology Diabetes and Metabolism Penile Induration Cell- and Tissue-Based Therapy Adipose tissue Matrix metalloproteinase Cell therapy Rats Sprague-Dawley Transforming Growth Factor beta1 Endocrinology & Metabolism Endocrinology Erectile Dysfunction Fibrosis medicine Animals Arterial Pressure RNA Messenger business.industry Penile Erection Stem Cells Tissue Inhibitor of Metalloproteinases medicine.disease Matrix Metalloproteinases Rats Disease Models Animal medicine.anatomical_structure Erectile dysfunction Reproductive Medicine Adipose Tissue Transcutaneous Electric Nerve Stimulation Cavernous Sinus Stem cell Peyronie's disease business Penis Stem Cell Transplantation |
| Zdroj: | Andrology. 2(2) |
| ISSN: | 2047-2927 |
| Popis: | Peyronie's disease (PD) is a localized connective tissue disorder that involves the tunica albuginea (TA) of the penis. While surgical correction remains the gold standard, the search for an effective and less invasive therapy continues. The objective of this study was to evaluate the effects of intratunical injection of adipose tissue-derived stem cells (ADSCs) for the prevention and treatment of erectile dysfunction in a rat model of PD. Twenty-four male Sprague-Dawley rats (300-350 g) were randomly divided into four groups: sham, PD, PD + ADSC (prevention) and PD + ADSC (treatment). All rats underwent penile injections into the TA with 50 μL vehicle (sham) or 0.5 μg transforming growth factor (TGF)-β1 (remaining groups). The ADSC groups received intratunical injections with 0.5 million rat-labelled ADSCs on day 0 (prevention) or day 30 (treatment). Forty-five days following TGF-β1 injection, rats underwent cavernous nerve stimulation (CNS) with total intracavernous-to-mean arterial pressure ratio (ICP/MAP) and total ICP recorded to measure response to therapy. Tissues were evaluated histologically and for mRNA expression of tissue inhibitors of metalloproteinases (TIMPs), matrix metalloproteinases (MMPs) and zymographic activity of MMPs. Statistical analysis was performed by analysis of variance followed by the Tukey test for post hoc comparisons. In both prevention and treatment groups, intratunical injection of ADSCs resulted in significantly higher ICP/MAP and total ICP in response to CNS compared with the PD group. Local injection of ADSCs prevented and/or reduced Peyronie's-like changes by decreasing the expression of TIMPs, and stimulating expression and activity of MMPs. This study documents the preventive and therapeutic benefits of ADSC on penile fibrosis and erectile function in an animal model of PD. |
| Databáze: | OpenAIRE |
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