Light-induced Nrf2−/− mice as atrophic age-related macular degeneration model and treatment with nanoceria laden injectable hydrogel

Autor: Kai Wang, Min Zheng, Kaitlyn Lee Lester, Zongchao Han
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Antioxidant
genetic structures
Light
medicine.medical_treatment
lcsh:Medicine
Diseases
medicine.disease_cause
Antioxidants
chemistry.chemical_compound
Glycols
Macular Degeneration
Mice
0302 clinical medicine
lcsh:Science
chemistry.chemical_classification
Mice
Knockout
Multidisciplinary
Hydrogels
Cerium
3. Good health
Cell biology
medicine.anatomical_structure
medicine.symptom
Biotechnology
Free Radicals
NF-E2-Related Factor 2
Inflammation
Article
Retina
03 medical and health sciences
Geographic Atrophy
medicine
Electroretinography
Animals
Reactive oxygen species
Chitosan
Retinal pigment epithelium
lcsh:R
Retinal
Macular degeneration
medicine.disease
eye diseases
Mice
Inbred C57BL
Oxidative Stress
030104 developmental biology
chemistry
Gene Expression Regulation
030221 ophthalmology & optometry
Nanoparticles
lcsh:Q
sense organs
Reactive Oxygen Species
Oxidative stress
Zdroj: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
ISSN: 2045-2322
Popis: Elevated oxidative stress and associated reactive oxygen species (ROS) accumulation are hallmarks in the induction and progression of age-related macular degeneration (AMD). By exposing nuclear factor erythroid 2-related factor (Nrf2) knockout (Nrf2−/−) mice to mild white light, we were able to generate a new dry-AMD like murine model to the study. This animal model developed phenotypes of photoreceptor degeneration, retinal function impairment, ROS accumulation, and inflammation reaction in a relatively shorter time. In the treatment of this animal model we utilized an antioxidative and water soluble nanoparticle known as glycol chitosan coated cerium oxide nanoparticles (GCCNP). The delivery of GCCNP protected retina against progressive retinal oxidative damage. Further combination of GCCNP with alginate-gelatin based injectable hydrogel provided synergistic antioxidant effects and achieved a more rapid recovery of the retinal pigment epithelium and photoreceptor cells. This combined treatment technique has the potential to translate into a clinical intervention for the treatment of AMD.
Databáze: OpenAIRE
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