Survival of Vibrio cholerae O1 on plastic materials

Autor: Eduardo Fernández-Escartín
Rok vydání: 1996
Předmět:
Zdroj: International Journal of Food Microbiology. 31:197-204
ISSN: 0168-1605
DOI: 10.1016/0168-1605(96)00985-3
Popis: Survival of environmental and clinical strains of Vibrio cholerae O1 was studied on glass and on two varieties of plastic materials. V. cholerae survived at least 2 days on glass, but was not recovered from polystyrene spoons after 15-20 min. Escherichia coli survived for at least 2 days on both glass slides and plastic spoons. Extracts, 10 and 50% (w/v) of ground plastic spoons in isotonic saline water, inactivated 10(4) vibrios in less than 2 h. Isotonic saline water rinses from polyethylene bags inactivated (0% survival) 15 out of 23 strains of V. cholerae in 1 h. A strain of V. cholerae (100-200 CFU/ml) directly suspended in 25 ml of isotonic saline per bag and maintained at 20 degrees C was progressively inactivated. The number of viable cells diminished 95% in 4 h in bags taken from non-sterile rolls. In sterile bags the decrease was 44% and 93% after 4 and 24 h, respectively. A variability up to 50% was observed in the antibacterial effect among the different bags, either sterile or taken from non-sterile rolls. These decreases in V. cholerae viability may result in false negative reports if water samples are collected and carried out in plastic bags.
Databáze: OpenAIRE