Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR
| Autor: | Byoung Chul Park, Jae Jong Kim, A. Young Kyoung, Si-Kyu Lim, Hyoung-Min Park, In Kyung Park |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
DNA polymerase
Molecular biology Science Single-nucleotide polymorphism Computational biology medicine.disease_cause Polymerase Chain Reaction Polymorphism Single Nucleotide Sensitivity and Specificity Article law.invention chemistry.chemical_compound law medicine SNP Humans Typing Polymerase chain reaction Cancer DNA Primers Mutation Multidisciplinary biology Oligonucleotide Biological techniques chemistry biology.protein Nucleic Acid Conformation Medicine DNA |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-7 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Genetic mutations such as single nucleotide polymorphisms (SNP) are known as one of the most common forms which related to various genetic disorders and cancers. Among of the methods developed for efficient detection of such SNP, polymerase chain reaction (PCR) methods are widely used worldwide for its cost and viable advantages. However, the technique to discriminate small amounts of SNP mixed in abundant normal DNA is incomplete due to intrinsic technical problems of PCR such as amplification occurring even in 3’mismatched cases because of high enzyme activity of DNA polymerases. To overcome the issue, specifically designed PCR platform, STexS (SNP typing with excellent specificity) using double stranded oligonucleotides was implemented as a means to emphasize the amplification of mutated SNP templates by decreasing unwanted amplification of 3’mismatched DNA copies. In this study, the results indicate several EGFR mutations were easily detected specifically utilizing the STexS platform. Further trials show the novel method works effectively to discriminate mutations in not only general allele specific (AS)-PCRs, but also amplification refractory mutation system (ARMS)-PCR. The STexS platform will give aid in PCRs targeting potential SNPs or genetically mutated biomarkers in human clinical samples. |
| Databáze: | OpenAIRE |
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