| Autor: |
Xue-Tao Pei, Wen Yue, Ya-Li Jia, Jun-Nian Zhou, Chao Zhai, Hong-Feng Yuan, Xin-Long Yan, Ji-Chao Du, Lin Chen, Quan Zeng, Jing-Xue Wang |
| Rok vydání: |
2023 |
| DOI: |
10.1158/1541-7786.22517046.v1 |
| Popis: |
PDF file - 2MB, Effect of SPINDLIN1 depletion on cancer cell proliferation and WNT/TCF-4 signaling. A. The siRNA interference sequence against SPINDLIN1 or an oligonucleotide control sequence was transfected into HeLa cells, and RT-PCR was used to examine the expression of SPINDLIN1, using �-ACTIN as an internal standard. B. HeLa cells treated with the same methods as A were used for the CCK-8 assay to analyze cell proliferation. The absorbance at 450 nm was monitored every 24 h over 7 d. N=6. C. Five hundred HeLa cells per well were treated with the same methods as A and were plated in 6-well plates and incubated for 2-3 weeks. Colonies were stained with crystal violet (top panels) and the numbers of colonies were counted (bottom panel). N=3, **p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
