Hair follicle changes following intense pulsed light axillary hair reduction: histometrical, histological and immunohistochemical evaluation
| Autor: | Selwet M. Saad, Hamza Abdel Raouf, Noha H. Moftah, Wael Hosam, Moetaz El-Domyati |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Adolescent Light medicine.medical_treatment Hair shaft Dermatology Intense pulsed light Hair Removal 030207 dermatology & venereal diseases 03 medical and health sciences Young Adult 0302 clinical medicine Proliferating Cell Nuclear Antigen Medicine Humans P53 expression integumentary system biology business.industry General Medicine Phototherapy Hair follicle Proliferating cell nuclear antigen medicine.anatomical_structure Ki-67 Antigen Morphometric analysis 030220 oncology & carcinogenesis Vellus hair biology.protein Immunohistochemistry Female sense organs Tumor Suppressor Protein p53 business Hair Follicle |
| Zdroj: | Archives of dermatological research. 309(3) |
| ISSN: | 1432-069X |
| Popis: | Intense pulsed light (IPL) has been used for years in hair reduction; however, no previous studies discussed quantitative histological and immunohistochemical changes of hair follicles after IPL. Accordingly, this study aims to objectively quantify histological and immunohistochemical changes of hair follicles after IPL hair reduction. Right axillae of 21 volunteers were subjected to 6 IPL sessions using Quanta system IPL and evaluated at 1 week and 1 month after last session (i.e., 3 and 4 months from the start of treatment, respectively) in comparison to baseline and left control axillae. Using hair count, histological and immunohistochemical assessment of vertical and serial transverse sections coupled with computerized morphometric analysis, determination of hair reduction percentage, measurement of hair shaft (HS) diameter, calculation of percentage of hair follicle types and quantitative evaluation of PCNA, Ki67 and P53 markers were performed. After IPL, there was significant decrease of hair count, HS diameter, percentage of terminal anagen follicles, terminal/vellus (T/V) ratio, anagen/telogen (A/T) ratio and expression of PCNA and Ki67; however, significant increase of percentage of terminal telogen and total vellus follicles with vellus-like type and P53 expression was identified. So, reduction of hair number and thickness occurred after IPL by induction of telogenesis and miniaturization through decreased hair follicle proliferation and increase in DNA damage that could favor apoptosis. |
| Databáze: | OpenAIRE |
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