Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers
| Autor: | Carlton L. Gyles, S. C. Whipp, C H Lee, Magdalene So, S L Moseley, H W Moon |
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| Rok vydání: | 1983 |
| Předmět: |
Bacterial Toxins
Immunology Biology medicine.disease_cause Microbiology Enterotoxins chemistry.chemical_compound Nucleic acid thermodynamics Enterotoxigenic Escherichia coli Escherichia coli medicine Amino Acid Sequence Cloning Molecular Gene Peptide sequence Base Sequence Toxin Escherichia coli Proteins Nucleic acid sequence Nucleic Acid Hybridization Molecular biology Phenotype Infectious Diseases Genes chemistry Genes Bacterial Parasitology DNA Research Article |
| Zdroj: | Infection and Immunity. 42:264-268 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.42.1.264-268.1983 |
| Popis: | The gene encoding heat-stable toxin II (STII) was cloned into an Escherichia coli K-12 strain, and its nucleotide sequence was determined. The deduced amino acid sequence indicates that STII is synthesized within the cell as a 71-amino-acid protein and that neither the DNA nor amino acid sequence bears any similarity to that of heat-stable toxin I. A DNA fragment containing the STII gene was used to probe enterotoxigenic E. coli clinical isolates with various toxin phenotypes and was shown to be useful in detecting all STII and only STII producers. |
| Databáze: | OpenAIRE |
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