Down-regulated gga-miR-223 inhibits proliferation and induces apoptosis of MG-infected DF-1 cells by targeting FOXO3
| Autor: | Yingfei Sun, Mengyun Zou, Xun Yin, Xiuli Peng, Yingjie Wang, Huanling Sun, Yun Han, Ronglong Luo |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
030106 microbiology Cell Mycoplasma gallisepticum Apoptosis Chick Embryo Biology Microbiology Fas ligand 03 medical and health sciences medicine Animals Cell Proliferation Cyclin-dependent kinase 1 Cell growth Fibroblasts Cell cycle Cell biology MicroRNAs 030104 developmental biology Infectious Diseases medicine.anatomical_structure FOXO3 biology.protein Cyclin-dependent kinase 6 Chickens |
| Zdroj: | Microbial Pathogenesis. 155:104927 |
| ISSN: | 0882-4010 |
| Popis: | BackgroundMycoplasma gallisepticum (MG) is a major poultry pathogen that can induce Chronic Respiratory Disease (CRD) in chickens, causing serious economic losses in poultry industry worldwide. Increasing evidence suggests that microRNAs (miRNAs) act as a vital role in resisting microbial pathogenesis and maintaining cellular mechanism. Our previous miRNAs sequencing data showed that gga-miR-223 expression level significantly decreased in MG-infected chicken lungs. The aim of this study was to reveal the role of gga-miR-223 in MG-induced CRD progression. ResultsWe found that gga-miR-223 was remarkably down regulated and forkhead box O3 (FOXO3) was up-regulated in both MG-infected chicken embryos lungs and the chicken embryonic fibroblast cell line (DF-1) by qPCR. FOXO3 was verified as the target gene of gga-miR-223 through bioinformatics analysis and dual-luciferase reporter assay. Further studies showed that overexpressed gga-miR-223 could promote cell proliferation, cell cycle, and inhibit cell apoptosis by notably promoting the expression of cell cycle marker genes cyclin-dependent kinase 1 (CDK1), cyclin-dependent kinase 6 (CDK6) and Cyclin D1 (CCND1) and inhibiting the expression of apoptosis markers Bcl-2-like 11(BIM), FAS ligand (FASLG) and TNF-related apoptosis-inducing ligand (TRAIL). As expected, FOXO3 knockdown group got similar results. Overexpression of gga-miR-223 observably promoted cell multiplication, cell cycle progression, and inhibited apoptosis of MG-infected DF-1 cells, while inhibited gga-miR-223 had the opposite effect. ConclusionsTaken together, upon MG-infection, downregulated gga-miR-223 could decrease proliferation, cycle progression, and increase apoptosis through directly targeting FOXO3 to exert an aggravating MG-infectious effect. |
| Databáze: | OpenAIRE |
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