CURRENT STRATEGIES FOR THE DETECTION OF MINIMAL RESIDUAL DISEASE IN CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA
| Autor: | Marcelo Eduardo de Lima Souza, Juliana Godoy Assumpção, S. Xavier, Mitiko Murao, Juliana Maria Camargos Rocha, Benigna Maria de Oliveira |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Oncology
medicine.medical_specialty medicine.medical_treatment Lymphoblastic Leukemia Gene Rearrangements of Ig/TCR 03 medical and health sciences 0302 clinical medicine Immunophenotyping Internal medicine medicine Review Articles Childhood Acute Lymphoblastic Leukemia Childhood all Minimal Residual Disease Chemotherapy business.industry lcsh:RC633-647.5 Cancer Hematology lcsh:Diseases of the blood and blood-forming organs medicine.disease Acute Lymphoblastic Leukemia Flow Cytometry Minimal residual disease Infectious Diseases Real-time polymerase chain reaction PCR 030220 oncology & carcinogenesis Immunology business 030215 immunology |
| Zdroj: | Mediterranean Journal of Hematology and Infectious Diseases, Vol 8, Iss 0, Pp e2016024-e2016024 (2016) Mediterranean Journal of Hematology and Infectious Diseases |
| ISSN: | 2035-3006 |
| Popis: | Acute lymphoblastic leukemia (ALL) is the most common cancer in children. Current treatment strategies for childhood ALL result in long term remission for approximately 90% of patients. However, therapeutic response is worse among those who relapse. Several risk stratification approaches based on clinical and biological aspects have been proposed in order to intensify treatment in patients with high risk of relapse and reduce toxicity on those with greater probability of cure.The detection of residual leukemic cells (minimal residual disease, MRD) is the most important prognostic factor to identify high risk patients, allowing redefinition of chemotherapy. In the last decades, several standardized research protocols evaluated MRD using immunophenotyping by flow cytometry and/or real time quantitative polymerase chain reaction at different time points during treatment. Both methods are highly sensitive (10-3 a 10-5), but expensive, complex, and, because of that, require qualified staff and frequently are restricted to reference centers.The aim of this article was to review technical aspects of immunophenotyping by flow cytometry and real time quantitative polymerase chain reaction to evaluate MRD in ALL. |
| Databáze: | OpenAIRE |
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