Analysis of cariogenic potential of alternative milk beverages by in vitro Streptococcus mutans biofilm model and ex vivo caries model
| Autor: | Qingzhao Yu, Tatyana Thompson, Zezhang T. Wen, Yan Huang, Janice A Townsend, Xiaoming Xu, Lin Zhu, Yapin Wang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Sucrose Dental Caries In Vitro Techniques Article Streptococcus mutans 03 medical and health sciences 0302 clinical medicine food Animals Food science Sugar Tooth Demineralization General Dentistry biology Enamel paint Chemistry Biofilm food and beverages 030206 dentistry Cell Biology General Medicine biology.organism_classification food.food Demineralization 030104 developmental biology Otorhinolaryngology Biofilms visual_art visual_art.visual_art_medium Fermentation Milk Substitutes Ex vivo Almond milk |
| Zdroj: | Arch Oral Biol |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2019.05.033 |
| Popis: | Objective To evaluate the potential of various alternative milk beverages to support bacterial biofilm formation and acid production and cause unbalanced demineralization. Design in vitro assays were used to examine the ability of the beverages to support Streptococcus mutans’ biofilm formation and acid production from sugar fermentation and the capacity of the beverages to buffer pH changes. Biofilm formation was done using 96-well plate model. Acid production was measured using L-Lactate assay kit, and the buffering capacity was assessed by pH titration. For ex vivo caries model, enamel and dentine slabs and S. mutans biofilms were exposed to selected alternative milk beverages three times a day, 30 min each, and by the end of the experiments, slab’s demineralization was assessed by loss of surface microhardness. Results Of the alternative milk beverages tested in this study, Original Almond consistently supported the most S. mutans biofilms, followed by Chocolate Cashew Milk, while the least biofilms were measured with Unsweetened Flax Milk. The most acids and the lowest culture pH were measured with Toasted Coconut Almond Milk, while the least buffering capacity was measured with Unsweetened Coconut Milk. The results of ex vivo caries model showed that like Bovine Whole Milk, repeated exposure to Original Almond led to significant enamel and dentine slab demineralization, when compared to those exposed to saline as a control (P Conclusions These results further provide support that popular alternative milk beverages, especially those with supplemental sugars, are potentially cariogenic. |
| Databáze: | OpenAIRE |
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