HilE is required for synergistic activation of SPI-1 gene expression in Salmonella enterica serovar Typhimurium
| Autor: | Mohamed Emara, James M. Slauch, Selwan Hamed, Christopher V. Rao, Riham M. Shawky |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Salmonella typhimurium Mutant lcsh:QR1-502 Repressor Gene Expression Biology Serogroup Microbiology lcsh:Microbiology 03 medical and health sciences Bacterial Proteins Salmonella Gene expression Gene 030304 developmental biology Regulation of gene expression 0303 health sciences SPI-1 030306 microbiology Activator (genetics) Acetate Wild type Promoter Gene Expression Regulation Bacterial Cell biology Gene regulation Repressor Proteins HilE Transcription Factors Research Article |
| Zdroj: | BMC Microbiology BMC Microbiology, Vol 21, Iss 1, Pp 1-9 (2021) |
| ISSN: | 1471-2180 |
| Popis: | BackgroundSalmonella entericaserovar Typhimurium is an intestinal pathogen capable of infecting a wide range of animals. It initiates infection by invading intestinal epithelial cells using a type III secretion system encoded withinSalmonellapathogenicity island 1 (SPI-1). The SPI-1 genes are regulated by multiple interacting transcription factors. The master regulator is HilD. HilE represses SPI-1 gene expression by binding HilD and preventing it from activating its target promoters. Previous work found that acetate and nutrients synergistically induce SPI-1 gene expression. In the present study, we investigated the role of HilE, nominally a repressor of SPI-1 gene expression, in mediating this response to acetate and nutrients.ResultsHilE is necessary for activation of SPI-1 gene expression by acetate and nutrients. In mutants lackinghilE, acetate and nutrients no longer increase SPI-1 gene expression but rather repress it. This puzzling response is not due to the BarA/SirA two component system, which governs the response to acetate. To identify the mechanism, we profiled gene expression using RNAseq in the wild type and a ΔhilEmutant under different growth conditions. Analysis of these data suggested that the Rcs system, which regulates gene expression in response to envelope stress, is involved. Consistent with this hypothesis, acetate and nutrients were able to induce SPI-1 gene expression in mutants lackinghilEand the Rcs system.ConclusionsWhile the exact mechanism is unknown, these results demonstrate the HilE, nominally a repressor of SPI-1 gene expression, can also function as an activator under the growth conditions investigated. Collectively, these results provide new insights regarding SPI-1 gene regulation and demonstrate that HilE is more complex than initially envisioned. |
| Databáze: | OpenAIRE |
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