DNA damage, DNA susceptibility to oxidation and glutathione redox status in patients with Alzheimer's disease treated with and without memantine
| Autor: | Gökhan Erkol, Çağlayan Akkaya, Yildiz Dincer, Hakan Yavuzer, Melda Bozluolcay, Serap Yavuzer |
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| Rok vydání: | 2017 |
| Předmět: |
inorganic chemicals
0301 basic medicine Male DNA damage medicine.drug_class Pharmacology medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Alzheimer Disease Memantine medicine Humans Aged Aged 80 and over DNA oxidation Glutathione Hydrogen Peroxide Middle Aged Molecular biology Comet assay Oxidative Stress 030104 developmental biology Neuroprotective Agents Treatment Outcome Neurology chemistry Acetylcholinesterase inhibitor Female Neurology (clinical) Cholinesterase Inhibitors Excitatory Amino Acid Antagonists Oxidation-Reduction 030217 neurology & neurosurgery DNA Oxidative stress medicine.drug DNA Damage |
| Zdroj: | Journal of the neurological sciences. 378 |
| ISSN: | 1878-5883 |
| Popis: | The aim of the current study was to compare oxidative DNA damage, DNA susceptibility to oxidation, and ratio of GSH/GSSG in patients with Alzheimer's disease (AD) treated with acetylcholinesterase inhibitor (AChEI) and combined AChEI+memantine. The study included 67 patients with AD and 42 volunteers as control. DNA damage parameters (strand breaks, oxidized purines, H2O2-induced DNA damage) in lymphocyte DNA and GSH/GSSG ratio in erythrocytes were determined by the comet assay and spectrophotometric assay, respectively. DNA damage was found to be higher, GSH/GSSG ratio was found to be lower in the AD group than those in the control group. DNA strand breaks and H2O2-induced DNA damage were lower in the patients taking AChEI+memantine than those in the patients taking AChEI but no significant difference was determined between the groups for oxidized purines and GSH/GSSG ratio. In conclusion, increased systemic oxidative DNA damage and DNA susceptibility to oxidation may be resulted from diminished GSH/GSSG ratio in AD patients. Although DNA strand breaks and H2O2-induced DNA damage are lower in the AD patients treated with combined AChEI and memantine, this may not indicate protective effect of memantine against DNA oxidation due to similar levels of oxidized purines in the patients treated with AChEI and AChEI+memantine. |
| Databáze: | OpenAIRE |
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