Effects of autologous SCF- and G-CSF-mobilized bone marrow stem cells on hypoxia-inducible factor-1 in rats with ischemia-reperfusion renal injury
| Autor: | B. Liang, Dasheng Yang, Shujun Li, J.L. Zhao, Lingyun Bi, J.G. Guo, H.T. Bai, Dean Zhao, R.X. Zhang |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Necrosis CD34 Stem cell factor Bone Marrow Cells Kidney Rats Sprague-Dawley chemistry.chemical_compound Random Allocation Internal medicine Granulocyte Colony-Stimulating Factor Genetics Medicine Animals Molecular Biology Erythropoietin Acute tubular necrosis Stem Cell Factor business.industry Bone Marrow Stem Cell General Medicine medicine.disease Hematopoietic Stem Cells Hypoxia-Inducible Factor 1 alpha Subunit Rats Vascular endothelial growth factor medicine.anatomical_structure Endocrinology chemistry Reperfusion Injury Immunology medicine.symptom business medicine.drug |
| Zdroj: | Genetics and molecular research : GMR. 14(2) |
| ISSN: | 1676-5680 |
| Popis: | To explore the mechanism whereby stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) jointly mobilize bone marrow stem cells (BMSCs) and promote kidney repair, male Sprague-Dawley rats were randomly assigned into 4 groups. In the treatment control group, rats were administered SCF (200 μg·kg(-1)·day(-1)) and G-CSF (50 μg·kg-1·day-1) for 5 days. In the treatment group, RIRI models were established, and 6 h later, SCF (200 μg·kg(-1)·day(-1)) and G-CSF (50 μg·kg(-1)·day(-1)) were administered for 5 days. In the model and treatment groups, tubular epithelial cell degeneration and necrosis were noticed, but the extent of repair in the treatment group was significantly better than in the model group. Five days after the operation, renal tissue CD34+ cells significantly increased in the model and treatment groups compared with the control and treatment control groups. HIF-1α, VEGF, and EPO expression in treatment groups increased significantly compared with the other groups. HIF- 1α, VEGF, EPO expression in the treatment control group increased significantly compared with the control group. Joint use of SCF and G-CSF increased the number of BMSCs in damaged kidney tissue and reduced the degree of renal tissue damage. BMSCs promote increased HIF-1α expression in renal tissue. Increased kidney tissue HIF- 1α and its target gene products VEGF and EPO expression possibly induce SCF and G-CSF to promote acute tubular necrosis repair. |
| Databáze: | OpenAIRE |
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