Comparative study of frequency of micronuclei in normal, potentially malignant diseases and oral squamous cell carcinoma
| Autor: | Varsha Ajit Sangle, Shobha C Bijjaragi, Suresh Kangane, Nishat Shah, Hrishikesh M Ghule, Sr Ashwini Rani |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Papanicolaou stain Context (language use) Micronuclei medicine.disease_cause General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine medicine Carcinogen smoking tobacco business.industry smokeless tobacco 030206 dentistry General Medicine potentially malignant diseases oral squamous cell carcinoma stomatognathic diseases 030104 developmental biology Smokeless tobacco Micronucleus test Biomarker (medicine) Original Article Chromosome breakage Carcinogenesis business |
| Zdroj: | Journal of Natural Science, Biology, and Medicine |
| ISSN: | 0976-9668 |
| DOI: | 10.4103/0976-9668.175049 |
| Popis: | Context: The assessment of micronuclei (MN) in exfoliated oral epithelial cells is a promising tool for the study of epithelial carcinogens and can be used to detect chromosome breakage or mitotic interference, thought to be relevant to carcinogenesis. Aims: To detect MN in exfoliated oral mucosal cells in individuals using various tobacco forms and also to detect frequency of MN in premalignant lesions and conditions (potentially malignant diseases [PMD's]) and oral squamous cell carcinoma (OSCC). To correlate frequency of MN in oral exfoliated cells in clinically diagnosed cases of OSCC followed by a histopathological grading. Materials and Methods: A total of 90 subjects (30 smokeless tobacco users, 30 smokers and 30 nontobacco users) consisted of clinically diagnosed cases of PMD's and OSCC were selected for the study. Cytosmears from the groups were stained with rapid Papanicolaou stain. MN was identified according to the Tolbert et al. criteria. Results: MN cells were found to be significantly higher in smokeless tobacco users than in smokers. The frequency of MN was three to four times higher in patients with OSCC as compared to patients in PMD's (P < 0.0001). The frequency of MN correlated with the histopathological grade was statistically significant. Conclusion: MN index can be used as a biomarker/screening test among the high-risk groups particularly the smokeless tobacco users and PMD's. MN can be a candidate to serve as a biomarker for prediction of the grade of OSCC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|