Calpain-3 is autolyzed and hence activated in human skeletal muscle 24 h following a single bout of eccentric exercise
| Autor: | Jason A. Bennie, Robyn M. Murphy, Michael J. McKenna, Murray J. Leikis, Graham D. Lamb, Craig A. Goodman |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors Adolescent Physiology Vastus lateralis muscle Muscle Proteins Physical exercise Isometric exercise Quadriceps Muscle Physiology (medical) Internal medicine medicine Eccentric Humans Exercise physiology Muscular dystrophy Creatine Kinase Exercise biology business.industry Calpain Skeletal muscle Anatomy medicine.disease medicine.anatomical_structure Endocrinology Torque biology.protein Creatine kinase Female business |
| Zdroj: | Journal of applied physiology (Bethesda, Md. : 1985). 103(3) |
| ISSN: | 8750-7587 |
| Popis: | The function and normal regulation of calpain-3, a muscle-specific Ca2+-dependent protease, is uncertain, although its absence leads to limb-girdle muscular dystrophy type 2A. This study examined the effect of eccentric exercise on calpain-3 autolytic activation, because such exercise is known to damage sarcomeric structures and to trigger adaptive changes that help prevent such damage on subsequent exercise. Six healthy human subjects performed a 30-min bout of one-legged, eccentric, knee extensor exercise. Torque measurements, vastus lateralis muscle biopsies, and venous blood samples were taken before and up to 7 days following the exercise. Peak isometric muscle torque was depressed immediately and at 3 h postexercise and recovered by 24 h, and serum creatine kinase concentration peaked at 24 h postexercise. The amount of autolyzed calpain-3 was unchanged immediately and 3 h after exercise, but increased markedly (from ∼16% to ∼35% of total) 24 h after the exercise, and returned to preexercise levels within 7 days. In contrast, the eccentric exercise produced little autolytic activation of the ubiquitous Ca2+-activated protease, μ-calpain. Eccentric exercise is the first physiological circumstance shown to result in calpain-3 activation in vivo. |
| Databáze: | OpenAIRE |
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