Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria
| Autor: | Liu Changmei, Jieyu Chu, Tingan Zhou, Yan Zhang, Yuechen Song, Shiyu Yu, Yifan Hu, Yijian Rao |
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| Rok vydání: | 2021 |
| Předmět: |
Endophytic bacteria
Hypha Bacillus Bioengineering In Vitro Techniques 010402 general chemistry Microbiology 01 natural sciences Applied Microbiology and Biotechnology Pigment Gene Expression Regulation Fungal Endophytes Leaf spot Secretion Bacillaceae Perylene Cercosporin Plant Diseases biology 010405 organic chemistry Chemistry Research biology.organism_classification Antimicrobial QR1-502 0104 chemical sciences visual_art visual_art.visual_art_medium Microbial fermentation Microbial Interactions Fermentation Co-culture Cercospora Bacteria Biotechnology |
| Zdroj: | Microbial Cell Factories Microbial Cell Factories, Vol 20, Iss 1, Pp 1-12 (2021) |
| ISSN: | 1475-2859 |
| Popis: | Background Owing to the excellent properties of photosensitization, cercosporin, one of naturally occurring perylenequinonoid pigments, has been widely used in photodynamic therapy, or as an antimicrobial agent and an organophotocatalyst. However, because of low efficiency of total chemical synthesis and low yield of current microbial fermentation, the limited production restricts its broad applications. Thus, the strategies to improve the production of cercosporin were highly desired. Besides traditional optimization methods, here we screened leaf-spot-disease-related endophytic bacteria to co-culture with our previous identified Cercospora sp. JNU001 to increase cercosporin production. Results Bacillus velezensis B04 and Lysinibacillus sp. B15 isolated from leaves with leaf spot diseases were found to facilitate cercosporin secretion into the broth and then enhance the production of cercosporin. After 4 days of co-culture, Bacillus velezensis B04 allowed to increase the production of cercosporin from 128.2 mg/L to 984.4 mg/L, which was 7.68-fold of the previously reported one. Lysinibacillus sp. B15 could also enhance the production of cercosporin with a yield of 626.3 mg/L, which was 4.89-fold higher than the starting condition. More importantly, we found that bacteria B04 and B15 employed two different mechanisms to improve the production of cercosporin, in which B04 facilitated cercosporin secretion into the broth by loosening and damaging the hyphae surface of Cercospora sp. JNU001 while B15 could adsorb cercosporin to improve its secretion. Conclusions We here established a novel and effective co-culture method to improve the production of cercosporin by increasing its secretion ability from Cercospora sp. JNU001, allowing to develop more potential applications of cercosporin. |
| Databáze: | OpenAIRE |
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