CHROMATO-MASS SPECTROMETRIC ANALYSIS OF URINE PROTEINS ASSOCIATED WITH THE FUNCTIONS OF TOLL-RECEPTORS IN A HEALTHY PERSON UNDER CONDITIONS OF 17-DAY ISOLATION
| Autor: | L Kh Pastyshkova, I N Goncharov, S A Ponomarev, A. G. Brzhozovsky, A. G. Goncharova, I M Larina, N B Zakharova, O L Morozova, D N Kashirina, K D Orlova |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Proteome Urine Urinalysis Mass Spectrometry 03 medical and health sciences 0302 clinical medicine medicine Humans FLNA Receptor MUC1 Chromatography Kidney Clusterin biology Chemistry Receptors IgG Biochemistry (medical) FCGR3A General Medicine Epithelium Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure Biochemistry biology.protein Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Russian Clinical Laboratory Diagnostics. 65:469-473 |
| ISSN: | 2412-1320 0869-2084 |
| Popis: | Under controlled conditions of 17-day isolation (Sirius-17 experiment), the protein composition of urine was studied in 6 healthy test volunteers-3 women and 3 men. Collection of samples in the form of a second freely separated morning urine fraction was carried out in the background (seven days before the experiment), as well as 1 day after the end of exposure. Chromatographic-mass-spectrometric semi-quantitative analysis of the protein composition of samples was performed on a system consisting of an Agilent 1100 chromatograph and an LTQ-FT Ultra hybrid mass spectrometer using bioinformatics resources UniProtKB, GeneOntology. An asymptomatic change in the immune defense system of kidney tissue after isolation in a closed hermetic object is associated with a change in the content of 7 proteins that provide functional activity of the TLR tubules of the kidneys - FcRIII, MUC1, Galectin-3, Ficolin-2, APOA1, FLNA, FCGR3A and Clusterin. These proteins are found to be useful biomarkers in the study of physiology and kidney diseases. They can be attributed to candidates for protein markers of the initial stages of impaired recognition by the epithelium of renal tubules of bacteria with known pathogenic potential. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|